Synonymous rpsH variants: the common denominator in Escherichia coli adapting to ionizing radiation

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Organisationseinheiten

Externe Organisationen

  • Medizinische Hochschule Hannover (MHH)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummerlqae110
FachzeitschriftNAR Genomics and Bioinformatics
Jahrgang6
Ausgabenummer3
Frühes Online-Datum24 Aug. 2024
PublikationsstatusVeröffentlicht - Sept. 2024

Abstract

Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional rpsH variants, potentially influences translational control of the ribosomal spc operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.

Zitieren

Synonymous rpsH variants: the common denominator in Escherichia coli adapting to ionizing radiation. / Stemwedel, Katharina; Haase, Nadin; Christ, Simon et al.
in: NAR Genomics and Bioinformatics, Jahrgang 6, Nr. 3, lqae110, 09.2024.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Stemwedel K, Haase N, Christ S, Bogdanova NV, Rudorf S. Synonymous rpsH variants: the common denominator in Escherichia coli adapting to ionizing radiation. NAR Genomics and Bioinformatics. 2024 Sep;6(3):lqae110. Epub 2024 Aug 24. doi: 10.1093/nargab/lqae110
Download
@article{c0e0899d5bbd4afcb99f91031b2a58c0,
title = "Synonymous rpsH variants: the common denominator in Escherichia coli adapting to ionizing radiation",
abstract = "Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional rpsH variants, potentially influences translational control of the ribosomal spc operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.",
author = "Katharina Stemwedel and Nadin Haase and Simon Christ and Natalia V Bogdanova and Sophia Rudorf",
note = "Publisher Copyright: {\textcopyright} 2024 The Author(s). Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics.",
year = "2024",
month = sep,
doi = "10.1093/nargab/lqae110",
language = "English",
volume = "6",
number = "3",

}

Download

TY - JOUR

T1 - Synonymous rpsH variants

T2 - the common denominator in Escherichia coli adapting to ionizing radiation

AU - Stemwedel, Katharina

AU - Haase, Nadin

AU - Christ, Simon

AU - Bogdanova, Natalia V

AU - Rudorf, Sophia

N1 - Publisher Copyright: © 2024 The Author(s). Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics.

PY - 2024/9

Y1 - 2024/9

N2 - Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional rpsH variants, potentially influences translational control of the ribosomal spc operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.

AB - Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional rpsH variants, potentially influences translational control of the ribosomal spc operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.

UR - http://www.scopus.com/inward/record.url?scp=85202454533&partnerID=8YFLogxK

U2 - 10.1093/nargab/lqae110

DO - 10.1093/nargab/lqae110

M3 - Article

VL - 6

JO - NAR Genomics and Bioinformatics

JF - NAR Genomics and Bioinformatics

IS - 3

M1 - lqae110

ER -

Von denselben Autoren