Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 718-728 |
| Seitenumfang | 11 |
| Fachzeitschrift | Journal of biotechnology |
| Jahrgang | 168 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - Dez. 2013 |
Abstract
Bottom-fermenting Saccharomyces pastorianus strains driving brewing fermentation processes are usually reused several times. It is still unclear, whether the number of successions may have an impact on cell physiology prompting consequences for brewing quality. In this study, fermentation performance of up to twenty consecutive runs in a brewery was investigated. For each run mRNA expression levels of cellular marker molecules, which are known to correlate with metabolism, hexose transport, aging processes, stress response mechanisms and flocculation capability was estimated to obtain information on changes in cell physiology over the successive runs. Low-density microarrays were used for this purpose and the resulting gene expression profiles were finally correlated with changes in the abiotic micro-environments. A surprising stability of the marker molecule expression profiles within each specific serial repitching was stated. Loss of flocculation or an advanced aging could not be detected during serial repitching in the analyzed brewery. However, certain runs of the serial repitchings showed high variation in stress response which was found to be caused by perturbations of the abiotic conditions. Regardless, the study showed that S. pastorianus can be used repeatedly in serial repitching processes without loss of prominent physiological characteristics.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Journal of biotechnology, Jahrgang 168, Nr. 4, 12.2013, S. 718-728.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Sustainability of industrial yeast serial repitching practice studied by gene expression and correlation analysis
AU - Bühligen, Franziska
AU - Rüdinger, Philipp
AU - Fetzer, Ingo
AU - Stahl, Frank
AU - Scheper, Thomas
AU - Harms, Hauke
AU - Müller, Susann
N1 - Funding information: F. B. was supported by the Scholarship Program of the German Federal Environmental Foundation (Grant number: AZ 20007/918 ). The work was supported by the Deutsche Brauwissenschaftsförderung (Grant number: R 422 ).
PY - 2013/12
Y1 - 2013/12
N2 - Bottom-fermenting Saccharomyces pastorianus strains driving brewing fermentation processes are usually reused several times. It is still unclear, whether the number of successions may have an impact on cell physiology prompting consequences for brewing quality. In this study, fermentation performance of up to twenty consecutive runs in a brewery was investigated. For each run mRNA expression levels of cellular marker molecules, which are known to correlate with metabolism, hexose transport, aging processes, stress response mechanisms and flocculation capability was estimated to obtain information on changes in cell physiology over the successive runs. Low-density microarrays were used for this purpose and the resulting gene expression profiles were finally correlated with changes in the abiotic micro-environments. A surprising stability of the marker molecule expression profiles within each specific serial repitching was stated. Loss of flocculation or an advanced aging could not be detected during serial repitching in the analyzed brewery. However, certain runs of the serial repitchings showed high variation in stress response which was found to be caused by perturbations of the abiotic conditions. Regardless, the study showed that S. pastorianus can be used repeatedly in serial repitching processes without loss of prominent physiological characteristics.
AB - Bottom-fermenting Saccharomyces pastorianus strains driving brewing fermentation processes are usually reused several times. It is still unclear, whether the number of successions may have an impact on cell physiology prompting consequences for brewing quality. In this study, fermentation performance of up to twenty consecutive runs in a brewery was investigated. For each run mRNA expression levels of cellular marker molecules, which are known to correlate with metabolism, hexose transport, aging processes, stress response mechanisms and flocculation capability was estimated to obtain information on changes in cell physiology over the successive runs. Low-density microarrays were used for this purpose and the resulting gene expression profiles were finally correlated with changes in the abiotic micro-environments. A surprising stability of the marker molecule expression profiles within each specific serial repitching was stated. Loss of flocculation or an advanced aging could not be detected during serial repitching in the analyzed brewery. However, certain runs of the serial repitchings showed high variation in stress response which was found to be caused by perturbations of the abiotic conditions. Regardless, the study showed that S. pastorianus can be used repeatedly in serial repitching processes without loss of prominent physiological characteristics.
KW - Process optimization
KW - Saccharomyces pastorianus
KW - Serial repitching
KW - Stress
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=84888847871&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2013.09.008
DO - 10.1016/j.jbiotec.2013.09.008
M3 - Article
C2 - 24060829
AN - SCOPUS:84888847871
VL - 168
SP - 718
EP - 728
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 4
ER -