Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 2686-2696 |
| Seitenumfang | 11 |
| Fachzeitschrift | Biomedical optics express |
| Jahrgang | 5 |
| Ausgabenummer | 8 |
| Frühes Online-Datum | 17 Juli 2014 |
| Publikationsstatus | Veröffentlicht - 1 Aug. 2014 |
| Extern publiziert | Ja |
Abstract
Gold nanoparticle mediated (GNOME) laser transfection/perforation fulfills the demands of a reliable transfection technique. It provides efficient delivery and has a negligible impact on cell viability. Furthermore, it reaches high-throughput applicability. However, currently only large gold particles (> 80 nm) allow successful GNOME laser perforation, probably due to insufficient sedimentation of smaller gold nanoparticles. The objective of this study is to determine whether this aspect can be addressed by a modification of silica particles with gold nanoparticles. Throughout the analysis, we show that after the attachment of gold nanoparticles to silica particles, comparable or better efficiencies to GNOME laser perforation are reached. In combination with 1 μm silica particles, we report laser perforation with gold nanoparticles with sizes down to 4 nm. Therefore, our investigations have great importance for the future research in and the fields of laser transfection combined with plasmonics.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
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in: Biomedical optics express, Jahrgang 5, Nr. 8, 01.08.2014, S. 2686-2696.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Surface modification of silica particles with gold nanoparticles as an augmentation of gold nanoparticle mediated laser perforation
AU - Kalies, Stefan
AU - Gentemann, Lara
AU - Schomaker, Markus
AU - Heinemann, Dag
AU - Ripken, Tammo
AU - Meyer, Heiko
PY - 2014/8/1
Y1 - 2014/8/1
N2 - Gold nanoparticle mediated (GNOME) laser transfection/perforation fulfills the demands of a reliable transfection technique. It provides efficient delivery and has a negligible impact on cell viability. Furthermore, it reaches high-throughput applicability. However, currently only large gold particles (> 80 nm) allow successful GNOME laser perforation, probably due to insufficient sedimentation of smaller gold nanoparticles. The objective of this study is to determine whether this aspect can be addressed by a modification of silica particles with gold nanoparticles. Throughout the analysis, we show that after the attachment of gold nanoparticles to silica particles, comparable or better efficiencies to GNOME laser perforation are reached. In combination with 1 μm silica particles, we report laser perforation with gold nanoparticles with sizes down to 4 nm. Therefore, our investigations have great importance for the future research in and the fields of laser transfection combined with plasmonics.
AB - Gold nanoparticle mediated (GNOME) laser transfection/perforation fulfills the demands of a reliable transfection technique. It provides efficient delivery and has a negligible impact on cell viability. Furthermore, it reaches high-throughput applicability. However, currently only large gold particles (> 80 nm) allow successful GNOME laser perforation, probably due to insufficient sedimentation of smaller gold nanoparticles. The objective of this study is to determine whether this aspect can be addressed by a modification of silica particles with gold nanoparticles. Throughout the analysis, we show that after the attachment of gold nanoparticles to silica particles, comparable or better efficiencies to GNOME laser perforation are reached. In combination with 1 μm silica particles, we report laser perforation with gold nanoparticles with sizes down to 4 nm. Therefore, our investigations have great importance for the future research in and the fields of laser transfection combined with plasmonics.
UR - http://www.scopus.com/inward/record.url?scp=84904659887&partnerID=8YFLogxK
U2 - 10.1364/BOE.5.002686
DO - 10.1364/BOE.5.002686
M3 - Article
AN - SCOPUS:84904659887
VL - 5
SP - 2686
EP - 2696
JO - Biomedical optics express
JF - Biomedical optics express
SN - 2156-7085
IS - 8
ER -