Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 025009 |
| Fachzeitschrift | BIOFABRICATION |
| Jahrgang | 9 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 28 Apr. 2017 |
| Extern publiziert | Ja |
Abstract
In the presented study, we have developed a synthetic strategy allowing a gradual variation of a polylactide arms' length, which later influences the micromorphology of the scaffold surface, formed by a two-photon polymerization technique. It has been demonstrated that the highest number of cells is present on the scaffolds with the roughest surface made of the polylactide with longer arms (PLA760), and osteogenic differentiation of mesenchymal stem cells is most pronounced on such scaffolds. According to the results of biological testing, the PLA760 scaffolds were implanted into a created cranial defect in a mouse for an in vivo assessment of the bone tissue formation. The in vivo experiments have shown that, by week 10, deposition of calcium phosphate particles occurs in the scaffold at the defect site, as well as, the formation of a new bone and ingrowth of blood vessels from the surrounding tissues. These results demonstrate that the cross-linked microstructured tetrafunctional polylactide scaffolds are promising microstructures for bone regeneration in tissue engineering.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Ingenieurwesen (insg.)
- Biomedizintechnik
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in: BIOFABRICATION, Jahrgang 9, Nr. 2, 025009, 28.04.2017.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Surface micromorphology of cross-linked tetrafunctional polylactide scaffolds inducing vessel growth and bone formation
AU - Kuznetsova, D.
AU - Ageykin, A.
AU - Koroleva, A.
AU - Deiwick, A.
AU - Shpichka, A.
AU - Solovieva, A.
AU - Kostjuk, S.
AU - Meleshina, A.
AU - Rodimova, S.
AU - Akovanceva, A.
AU - Butnaru, D.
AU - Frolova, A.
AU - Zagaynova, E.
AU - Chichkov, B.
AU - Bagratashvili, V.
AU - Timashev, P.
PY - 2017/4/28
Y1 - 2017/4/28
N2 - In the presented study, we have developed a synthetic strategy allowing a gradual variation of a polylactide arms' length, which later influences the micromorphology of the scaffold surface, formed by a two-photon polymerization technique. It has been demonstrated that the highest number of cells is present on the scaffolds with the roughest surface made of the polylactide with longer arms (PLA760), and osteogenic differentiation of mesenchymal stem cells is most pronounced on such scaffolds. According to the results of biological testing, the PLA760 scaffolds were implanted into a created cranial defect in a mouse for an in vivo assessment of the bone tissue formation. The in vivo experiments have shown that, by week 10, deposition of calcium phosphate particles occurs in the scaffold at the defect site, as well as, the formation of a new bone and ingrowth of blood vessels from the surrounding tissues. These results demonstrate that the cross-linked microstructured tetrafunctional polylactide scaffolds are promising microstructures for bone regeneration in tissue engineering.
AB - In the presented study, we have developed a synthetic strategy allowing a gradual variation of a polylactide arms' length, which later influences the micromorphology of the scaffold surface, formed by a two-photon polymerization technique. It has been demonstrated that the highest number of cells is present on the scaffolds with the roughest surface made of the polylactide with longer arms (PLA760), and osteogenic differentiation of mesenchymal stem cells is most pronounced on such scaffolds. According to the results of biological testing, the PLA760 scaffolds were implanted into a created cranial defect in a mouse for an in vivo assessment of the bone tissue formation. The in vivo experiments have shown that, by week 10, deposition of calcium phosphate particles occurs in the scaffold at the defect site, as well as, the formation of a new bone and ingrowth of blood vessels from the surrounding tissues. These results demonstrate that the cross-linked microstructured tetrafunctional polylactide scaffolds are promising microstructures for bone regeneration in tissue engineering.
KW - Bone formation
KW - Crosslinked tetrafunctional polylactide
KW - Scaffolds
KW - Surface micromorphology
KW - Two-photon polymerization
KW - Vessel growth
UR - http://www.scopus.com/inward/record.url?scp=85021261505&partnerID=8YFLogxK
U2 - 10.1088/1758-5090/aa6725
DO - 10.1088/1758-5090/aa6725
M3 - Article
C2 - 28300041
AN - SCOPUS:85021261505
VL - 9
JO - BIOFABRICATION
JF - BIOFABRICATION
SN - 1758-5082
IS - 2
M1 - 025009
ER -