TY - GEN

T1 - Surface enhanced Raman spectroscopy based analysis of SARS-CoV-2 spike protein binding to ACE2 receptor

AU - Wetzel, Christoph

AU - Jansen-Olliges, Linda

AU - Zeilinger, Carsten

AU - Surup, Frank

AU - Stadler, Marc

AU - Roth, Bernhard

N1 - Funding Information: The authors thank the German Federal Ministry of Education and Research (BMBF) for supporting the research (grant ref. 13N15259). We also acknowledge the support by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453) and by FOR 5170: CytoLabs - Systematische Untersuchung und Ausbeutung von Cytochalasanen.

PY - 2023/3/15

Y1 - 2023/3/15

N2 - The SARS-CoV-2 virus is still a challenge because of its diversity and mutations. The binding interactions of the angiotensin converting enzyme 2 (ACE2) receptor and the spike protein are relevant for the SARS-CoV-2 virus to enter the cell. Consequently, it is important and helpful to analyze binding activities and the changes in the structure of the ACE2 receptor and the spike protein. Surface enhanced Raman spectroscopy is able to analyze small concentrations of the proteins without contact, non-invasively and label-free. In this work, we present a SERS based approach in the visible wavelength range to analyze and study the binding interactions of the ACE2 receptor and the spike protein. SERS measurements of the ACE2 receptor, the spike protein and the ACE2-spike complex were performed. Additionally, an inhibitor was used to prevent the spike protein from binding to ACE2 and to compare the results. The analysis of the measured SERS spectra reveals structural differences and changes due to binding activities. Thus, we show that the performed SERS based approach can help for rapid and non-invasive analysis of binding interactions of the ACE2-spike complex and also of protein binding in general.

AB - The SARS-CoV-2 virus is still a challenge because of its diversity and mutations. The binding interactions of the angiotensin converting enzyme 2 (ACE2) receptor and the spike protein are relevant for the SARS-CoV-2 virus to enter the cell. Consequently, it is important and helpful to analyze binding activities and the changes in the structure of the ACE2 receptor and the spike protein. Surface enhanced Raman spectroscopy is able to analyze small concentrations of the proteins without contact, non-invasively and label-free. In this work, we present a SERS based approach in the visible wavelength range to analyze and study the binding interactions of the ACE2 receptor and the spike protein. SERS measurements of the ACE2 receptor, the spike protein and the ACE2-spike complex were performed. Additionally, an inhibitor was used to prevent the spike protein from binding to ACE2 and to compare the results. The analysis of the measured SERS spectra reveals structural differences and changes due to binding activities. Thus, we show that the performed SERS based approach can help for rapid and non-invasive analysis of binding interactions of the ACE2-spike complex and also of protein binding in general.

KW - ACE2

KW - Protein analysis

KW - SARS-CoV2 spike protein

KW - Surface enhanced Raman spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85159653105&partnerID=8YFLogxK

U2 - 10.1117/12.2648954

DO - 10.1117/12.2648954

M3 - Conference contribution

AN - SCOPUS:85159653105

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI

A2 - Tarnok, Attila

A2 - Houston, Jessica P.

PB - SPIE

T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023

Y2 - 30 January 2023 through 1 February 2023

ER -