Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | 2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011 |
| Publikationsstatus | Veröffentlicht - 1 Feb. 2011 |
| Veranstaltung | 2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011 - Munich, Deutschland Dauer: 22 Mai 2011 → 26 Mai 2011 |
Publikationsreihe
| Name | 2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011 |
|---|
Abstract
Multiphoton fluorescence microscopy based on femtosecond laser scanning is a powerful technique for three dimensional optical sectioning in life sciences. The method is based on the simultaneous absorption of two or three photons in the focal volume of a high NA microscope objective. The same setup is suited for nanodissection of living cells and subcellular structures. A very small lateral extent of the modified focal volume is required to minimize collateral damage in the vicinity of the laser focus and to improve long-term cell viability. By using high NA microscope objectives and laser pulse energies close to the ablation threshold, the lateral extent of the modified material is limited to less than 1 m. This diffraction limited resolution can be further improved by techniques generally referred to as superresolution. These are achieved by controlling the phase of the laser beam with a diffractive filter placed at the exit pupil of an optical system. We integrated this technique into nanosurgery of cells, which we demonstrate here for the first time.
ASJC Scopus Sachgebiete
- Ingenieurwesen (insg.)
- Elektrotechnik und Elektronik
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2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011. 2011. 5943235 (2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Superresolved femtosecond laser nanosurgery of cells
AU - Pospiech, Matthias
AU - Emons, Moritz
AU - Kuetemeyer, Kai
AU - Heisterkamp, Alexander
AU - Morgner, Uwe
PY - 2011/2/1
Y1 - 2011/2/1
N2 - Multiphoton fluorescence microscopy based on femtosecond laser scanning is a powerful technique for three dimensional optical sectioning in life sciences. The method is based on the simultaneous absorption of two or three photons in the focal volume of a high NA microscope objective. The same setup is suited for nanodissection of living cells and subcellular structures. A very small lateral extent of the modified focal volume is required to minimize collateral damage in the vicinity of the laser focus and to improve long-term cell viability. By using high NA microscope objectives and laser pulse energies close to the ablation threshold, the lateral extent of the modified material is limited to less than 1 m. This diffraction limited resolution can be further improved by techniques generally referred to as superresolution. These are achieved by controlling the phase of the laser beam with a diffractive filter placed at the exit pupil of an optical system. We integrated this technique into nanosurgery of cells, which we demonstrate here for the first time.
AB - Multiphoton fluorescence microscopy based on femtosecond laser scanning is a powerful technique for three dimensional optical sectioning in life sciences. The method is based on the simultaneous absorption of two or three photons in the focal volume of a high NA microscope objective. The same setup is suited for nanodissection of living cells and subcellular structures. A very small lateral extent of the modified focal volume is required to minimize collateral damage in the vicinity of the laser focus and to improve long-term cell viability. By using high NA microscope objectives and laser pulse energies close to the ablation threshold, the lateral extent of the modified material is limited to less than 1 m. This diffraction limited resolution can be further improved by techniques generally referred to as superresolution. These are achieved by controlling the phase of the laser beam with a diffractive filter placed at the exit pupil of an optical system. We integrated this technique into nanosurgery of cells, which we demonstrate here for the first time.
UR - http://www.scopus.com/inward/record.url?scp=80052285640&partnerID=8YFLogxK
U2 - 10.1109/CLEOE.2011.5943235
DO - 10.1109/CLEOE.2011.5943235
M3 - Conference contribution
AN - SCOPUS:80052285640
SN - 9781457705335
T3 - 2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011
BT - 2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011
T2 - 2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011
Y2 - 22 May 2011 through 26 May 2011
ER -