Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Xing Xie
  • Siwen Wang
  • Sunny C. Jiang
  • Janina Bahnemann
  • Michael R. Hoffmann

Organisationseinheiten

Externe Organisationen

  • University of California at Irvine
  • California Institute of Technology (Caltech)
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Details

OriginalspracheEnglisch
Seiten (von - bis)57-61
Seitenumfang5
FachzeitschriftEnvironmental Science and Technology Letters
Jahrgang3
Ausgabenummer2
PublikationsstatusVeröffentlicht - 5 Jan. 2016

Abstract

Polymerase chain reaction (PCR)-based methods have been developed and increasingly used for rapid and sensitive detection of pathogens in water samples to better protect public health. A propidium monoazide (PMA) pretreatment can help to differentiate between viable and dead cells, but the photoactivation of PMA normally requires the use of an energy-consuming halogen light, which is not suitable for off-the-grid applications. Herein, we investigate sunlight as an alternative light source. Our results suggest that sunlight can successfully activate PMA, and the sunlight-activated PMA pretreatment can effectively reduce the amplification of DNA derived from dead cells in PCR assays. Potentially, a sunlight-activated PMA pretreatment unit can be integrated into a lab-on-a-chip PCR device for off-the-grid microbial detection and quantification.

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Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction. / Xie, Xing; Wang, Siwen; Jiang, Sunny C. et al.
in: Environmental Science and Technology Letters, Jahrgang 3, Nr. 2, 05.01.2016, S. 57-61.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Xie, X, Wang, S, Jiang, SC, Bahnemann, J & Hoffmann, MR 2016, 'Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction', Environmental Science and Technology Letters, Jg. 3, Nr. 2, S. 57-61. https://doi.org/10.1021/acs.estlett.5b00348
Xie, X., Wang, S., Jiang, S. C., Bahnemann, J., & Hoffmann, M. R. (2016). Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction. Environmental Science and Technology Letters, 3(2), 57-61. https://doi.org/10.1021/acs.estlett.5b00348
Xie X, Wang S, Jiang SC, Bahnemann J, Hoffmann MR. Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction. Environmental Science and Technology Letters. 2016 Jan 5;3(2):57-61. doi: 10.1021/acs.estlett.5b00348
Xie, Xing ; Wang, Siwen ; Jiang, Sunny C. et al. / Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction. in: Environmental Science and Technology Letters. 2016 ; Jahrgang 3, Nr. 2. S. 57-61.
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abstract = "Polymerase chain reaction (PCR)-based methods have been developed and increasingly used for rapid and sensitive detection of pathogens in water samples to better protect public health. A propidium monoazide (PMA) pretreatment can help to differentiate between viable and dead cells, but the photoactivation of PMA normally requires the use of an energy-consuming halogen light, which is not suitable for off-the-grid applications. Herein, we investigate sunlight as an alternative light source. Our results suggest that sunlight can successfully activate PMA, and the sunlight-activated PMA pretreatment can effectively reduce the amplification of DNA derived from dead cells in PCR assays. Potentially, a sunlight-activated PMA pretreatment unit can be integrated into a lab-on-a-chip PCR device for off-the-grid microbial detection and quantification.",
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note = "Funding information: This work was supported by National Research Foundation of Korea (NRF) grant funded by Korea government (MSIP) (No. 2013M2A2A7043663). This work was supported by National Research Foundation of Korea (NRF) grant funded by Korea government (MSIP) (No. 2013M2A2A7043663).",
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AU - Xie, Xing

AU - Wang, Siwen

AU - Jiang, Sunny C.

AU - Bahnemann, Janina

AU - Hoffmann, Michael R.

N1 - Funding information: This work was supported by National Research Foundation of Korea (NRF) grant funded by Korea government (MSIP) (No. 2013M2A2A7043663). This work was supported by National Research Foundation of Korea (NRF) grant funded by Korea government (MSIP) (No. 2013M2A2A7043663).

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