Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 004871 |
| Fachzeitschrift | International Journal of Systematic and Evolutionary Microbiology |
| Jahrgang | 71 |
| Ausgabenummer | 7 |
| Publikationsstatus | Veröffentlicht - 8 Juli 2021 |
| Extern publiziert | Ja |
Abstract
A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany. Cells of BGR 140T were Gram-stain-positive, endospore-forming, motile and rod-shaped. BGR 140T grew aerobically at 25–55 °C (optimum 45 °C) and at pH 1.5–5.0 (optimum pH 3.0). The results of analysis of the 16S rRNA gene sequences indi-cated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6%, respectively. Its cell wall peptidoglycan is A1γ, composed of meso-diaminopimelic acid. The res-piratory quinone is DMK-6. The major polar lipids were determined to be glycolipid, phospholipid and phosphatidylglycerol. The predominant fatty acid is 11-cycloheptanoyl-undecanoate. The genomic DNA G+C content is 58.2 mol%. On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp. nov. is proposed because of its origin. Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Mikrobiologie
- Agrar- und Biowissenschaften (insg.)
- Ökologie, Evolution, Verhaltenswissenschaften und Systematik
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in: International Journal of Systematic and Evolutionary Microbiology, Jahrgang 71, Nr. 7, 004871, 08.07.2021.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Sulfobacillus harzensis sp. Nov., an acidophilic bacterium inhabiting mine tailings from a polymetallic mine
AU - Zhang, Ruiyong
AU - Hedrich, Sabrina
AU - Jin, Decai
AU - Breuker, Anja
AU - Schippers, Axel
N1 - Funding Information: The study was supported by the German Science Foundation (DFG grant SCHI 535/15–1). The TEM analysis was supported by the National Natural Science Foundation of China (No. 42076044).
PY - 2021/7/8
Y1 - 2021/7/8
N2 - A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany. Cells of BGR 140T were Gram-stain-positive, endospore-forming, motile and rod-shaped. BGR 140T grew aerobically at 25–55 °C (optimum 45 °C) and at pH 1.5–5.0 (optimum pH 3.0). The results of analysis of the 16S rRNA gene sequences indi-cated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6%, respectively. Its cell wall peptidoglycan is A1γ, composed of meso-diaminopimelic acid. The res-piratory quinone is DMK-6. The major polar lipids were determined to be glycolipid, phospholipid and phosphatidylglycerol. The predominant fatty acid is 11-cycloheptanoyl-undecanoate. The genomic DNA G+C content is 58.2 mol%. On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp. nov. is proposed because of its origin. Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
AB - A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany. Cells of BGR 140T were Gram-stain-positive, endospore-forming, motile and rod-shaped. BGR 140T grew aerobically at 25–55 °C (optimum 45 °C) and at pH 1.5–5.0 (optimum pH 3.0). The results of analysis of the 16S rRNA gene sequences indi-cated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6%, respectively. Its cell wall peptidoglycan is A1γ, composed of meso-diaminopimelic acid. The res-piratory quinone is DMK-6. The major polar lipids were determined to be glycolipid, phospholipid and phosphatidylglycerol. The predominant fatty acid is 11-cycloheptanoyl-undecanoate. The genomic DNA G+C content is 58.2 mol%. On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp. nov. is proposed because of its origin. Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
KW - Acidophiles
KW - Biomining
KW - Iron-and sulfur-oxidation
KW - Mine tailings
KW - Sulfobacillus
UR - http://www.scopus.com/inward/record.url?scp=85110998210&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004871
DO - 10.1099/ijsem.0.004871
M3 - Article
C2 - 34236956
AN - SCOPUS:85110998210
VL - 71
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 7
M1 - 004871
ER -