TY - JOUR

T1 - Succession of the soil bacterial community as resource utilization shifts from plant residues to rhizodeposits

AU - Fu, Yingyi

AU - Luo, Yu

AU - Tang, Caixian

AU - Li, Yong

AU - Guggenberger, Georg

AU - Xu, Jianming

N1 - Funding Information: This study was supported by the National Science Foundation of China ( U1901601 ) and Zhejiang Provincial Natural Science Foundation of China under Grant Number of R19D010005 .

PY - 2022/10

Y1 - 2022/10

N2 - Identifying the core taxa involved in the utilization of plant straw or/and rhizodeposits is key to understanding the microbial mechanisms underlying the turnover of these plant-derived organic matters, known as the “brown” path and “green” path, respectively. However, this still remains a challenge, primarily due to methodological limitations. By adopting a novel experimental design of parallel 13C-labelling and DNA-SIP, we identified microorganisms that utilized rhizodeposits and plant straw in soils containing these two plant-derived substances. Additionally, to compare straw utilizers in the soil without plants (straw only) and with plants (straw plus rhizodeposits), we investigated the shift of these substrate-stimulated communities (e.g., straw utilizers) in the presence of live maize plants. Here we showed that i) rhizodeposits were used by a wide range of root-associated microorganisms but plant straw was mainly utilized by oligotrophs, e.g., Arthrobacter, when these two plant-derived substances were co-present; and ii) there was a shift in the bacterial straw utilizer community, e.g., Streptomyces, and their physiological metabolism from saprotrophy to symbiosis when maize plants were present. This study identified distinct utilizers of plant-derived substances, i.e. straw or rhizodeposits, and revealed the shift of soil bacterial community and metabolisms along the autotroph -saprotroph -mutualism continuum.

AB - Identifying the core taxa involved in the utilization of plant straw or/and rhizodeposits is key to understanding the microbial mechanisms underlying the turnover of these plant-derived organic matters, known as the “brown” path and “green” path, respectively. However, this still remains a challenge, primarily due to methodological limitations. By adopting a novel experimental design of parallel 13C-labelling and DNA-SIP, we identified microorganisms that utilized rhizodeposits and plant straw in soils containing these two plant-derived substances. Additionally, to compare straw utilizers in the soil without plants (straw only) and with plants (straw plus rhizodeposits), we investigated the shift of these substrate-stimulated communities (e.g., straw utilizers) in the presence of live maize plants. Here we showed that i) rhizodeposits were used by a wide range of root-associated microorganisms but plant straw was mainly utilized by oligotrophs, e.g., Arthrobacter, when these two plant-derived substances were co-present; and ii) there was a shift in the bacterial straw utilizer community, e.g., Streptomyces, and their physiological metabolism from saprotrophy to symbiosis when maize plants were present. This study identified distinct utilizers of plant-derived substances, i.e. straw or rhizodeposits, and revealed the shift of soil bacterial community and metabolisms along the autotroph -saprotroph -mutualism continuum.

KW - Bacterial life strategy

KW - ContinuousCO labeling

KW - DNA-SIP

KW - Keystone microbiota

KW - Plant-derived carbon

UR - http://www.scopus.com/inward/record.url?scp=85135706624&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2022.108785

DO - 10.1016/j.soilbio.2022.108785

M3 - Article

AN - SCOPUS:85135706624

VL - 173

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

M1 - 108785

ER -