TY - JOUR

T1 - Succession of bacterial community and methanotrophy during lake shrinkage

AU - Mo, Yongliang

AU - Jin, Feng

AU - Zheng, Yan

AU - Baoyin, Taogetao

AU - Ho, Adrian

AU - Jia, Zhongjun

N1 - Funding Information: The work was financially supported by the National Natural Science Foundation of China (41701302, 91751204), the Strategic Priority Research Program of Chinese Academy of Sciences (XDB15040000), and the open fund for the State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences (Y812000008). Financial support for AH is provided by the Deutsche Forschungsgemeinschaft grant (DFG HO6234/1-1) and the Leibniz Universität Hannover, Germany. Acknowledgments Funding Information: We would like to show our gratitude to Mrs. Yufang Sun from the analysis center of Institute of Soil Science and to Prof. Shenqiang Wang, Prof. Weixin Ding, Dr. Yi Cheng, Dr. Tiehu He and Dr. Xian Xiao from the Institute of Soil Science for their assistance in analyzing soil, gas samples and SEM construction. We also thank Ms. Liyan Zhang, Ms. Kunkun Fan, Ms. Maomao Feng, and Mr. Jie Liu, Mr. Zhiying Guo for the help in bioinformatic analysis on the China Soil Microbiome Data Platform (http://159.226.101.185/microbe/). Our lab colleagues are gratefully acknowledged for technical assistance and helpful discussion.

PY - 2020/3

Y1 - 2020/3

N2 - Purpose: The shrinkage of vast inland lakes affects microbially mediated soil biogeochemical processes, which are critical for maintaining ecosystem sustainability, such as microbial diversity and a balanced CH4 budget. Here we aimed to elucidate shifts in the bacterial community and methanotrophy during the shrinkage of a saline lake. Materials and methods: Sediments and soils along a gradient transecting a saline lake, saline riparian land, and grassland were collected. The succession of microbial communities was characterized by high-throughput sequencing of the V4-V5 region of 16S rRNA genes coupled to non-metric multidimensional scaling (NMDS), linear discriminant effect size (LEfSe), community assembly, and co-occurrence network analyses. We further incubated these samples under a 10% CH4 (v/v) atmospheric condition to determine the response of methane oxidation potentials and of methanotrophs to lake shrinkage by using pmoA-based qPCR and amplicon sequencing. Results and discussion: LEfSe and NMDS analyses showed significant differences in bacterial communities among 3 stages of lake shrinkage. The microbial taxa with the highest increase were phylogenetically affiliated with unclassified Rhizobiales, Panacagrimonas, and Pseudomonas in saline and grassland soils when compared with sediments. Microbial community assembly was largely determined by deterministic rather than stochastic processes (NTI > 2). The drastic increase of Methylocystis-like (type II) methanotrophs was observed during lake shrinkage, while type I methanotrophs showed a decreasing trend. However, upon consuming high-concentration methane of about 10%, type I methanotrophs dominated methane-oxidizing communities in lake sediment (Methylomonas), riparian saline soil (Methylomicrobium), and grassland soil (Methylobacter). Structural equation model identified soil pH, C/N ratio, and soil texture as key factors affecting methane oxidation rates and the methanotrophic community. Conclusions: Lake shrinkage showed profound impacts on the overall bacterial communities and methane oxidizers. Soil physico-chemical properties likely shaped the bacterial community and phylogenetically distinct methanotrophs during lake shrinkage.

AB - Purpose: The shrinkage of vast inland lakes affects microbially mediated soil biogeochemical processes, which are critical for maintaining ecosystem sustainability, such as microbial diversity and a balanced CH4 budget. Here we aimed to elucidate shifts in the bacterial community and methanotrophy during the shrinkage of a saline lake. Materials and methods: Sediments and soils along a gradient transecting a saline lake, saline riparian land, and grassland were collected. The succession of microbial communities was characterized by high-throughput sequencing of the V4-V5 region of 16S rRNA genes coupled to non-metric multidimensional scaling (NMDS), linear discriminant effect size (LEfSe), community assembly, and co-occurrence network analyses. We further incubated these samples under a 10% CH4 (v/v) atmospheric condition to determine the response of methane oxidation potentials and of methanotrophs to lake shrinkage by using pmoA-based qPCR and amplicon sequencing. Results and discussion: LEfSe and NMDS analyses showed significant differences in bacterial communities among 3 stages of lake shrinkage. The microbial taxa with the highest increase were phylogenetically affiliated with unclassified Rhizobiales, Panacagrimonas, and Pseudomonas in saline and grassland soils when compared with sediments. Microbial community assembly was largely determined by deterministic rather than stochastic processes (NTI > 2). The drastic increase of Methylocystis-like (type II) methanotrophs was observed during lake shrinkage, while type I methanotrophs showed a decreasing trend. However, upon consuming high-concentration methane of about 10%, type I methanotrophs dominated methane-oxidizing communities in lake sediment (Methylomonas), riparian saline soil (Methylomicrobium), and grassland soil (Methylobacter). Structural equation model identified soil pH, C/N ratio, and soil texture as key factors affecting methane oxidation rates and the methanotrophic community. Conclusions: Lake shrinkage showed profound impacts on the overall bacterial communities and methane oxidizers. Soil physico-chemical properties likely shaped the bacterial community and phylogenetically distinct methanotrophs during lake shrinkage.

KW - CH oxidation

KW - Community assembly

KW - High-throughput sequencing

KW - Lake shrinkage

KW - Methanotroph

UR - http://www.scopus.com/inward/record.url?scp=85081322771&partnerID=8YFLogxK

U2 - 10.1007/s11368-019-02465-6

DO - 10.1007/s11368-019-02465-6

M3 - Article

AN - SCOPUS:85081322771

VL - 20

SP - 1545

EP - 1557

JO - Journal of soils and sediments

JF - Journal of soils and sediments

SN - 1439-0108

IS - 3

ER -