TY - JOUR

T1 - Structural damage of Bacillus subtilis biofilms using pulsed laser interaction with gold thin films

AU - Krawinkel, Judith

AU - Torres, Maria Leilani

AU - Mhatre, Eisha

AU - Kovács, Ákos T.

AU - Heisterkamp, Alexander

N1 - Funding Information: We acknowledge Tetyana Melnyk for helping us in the SEM imaging. We thank Tobias Birr for the helpful discussions on calculations of gold film thickness. We are grateful to Coherent GmbH for lending us the Helios laser. We also thank Prof. Kürsad Turgay and Heinrich Schäffer for providing additional samples. Work in the laboratory of A. T. K. is supported by Grants KO4741/2-1 and KO4741/3-1 from the Deutsche Forschungsgemeinschaft (DFG). E. M. was supported by Jena School for Microbial Communications (JSMC). The LSM780 microscope was financed by a grant from Thüringer Ministerium für Bildung, Wissenschaft und Kultur (project B11024-715). A.H acknowledges the financial support of Excellence Cluster, REBIRTH and the funding initiative “Biofabrication for NIFE”.

PY - 2017/8/11

Y1 - 2017/8/11

N2 - There is a huge interest in developing strategies to effectively eliminate biofilms due to their negative impact in both industrial and clinical settings. In this study, structural damage was induced on two day-old B. subtilis biofilms using the interaction of 532 nm pulsed laser with gold thin films. Radiant exposure of 225 mJ/cm2 induced distinct changes on the surface structure and overall morphology of the matured biofilms after laser irradiation. Moreover, at the radiant exposure used, changes in the colour and viscosity of the biofilm were observed which may indicate a compromised extracellular matrix. Irradiated biofilms in the presence of gold film also showed strong propidium iodide signal which implies an increase in the number of dead bacterial cells after laser treatment. Thus, this laser-based technique is a promising approach in targeting and eradicating matured biofilms attached on surfaces such as medical implants. (Figure presented.).

AB - There is a huge interest in developing strategies to effectively eliminate biofilms due to their negative impact in both industrial and clinical settings. In this study, structural damage was induced on two day-old B. subtilis biofilms using the interaction of 532 nm pulsed laser with gold thin films. Radiant exposure of 225 mJ/cm2 induced distinct changes on the surface structure and overall morphology of the matured biofilms after laser irradiation. Moreover, at the radiant exposure used, changes in the colour and viscosity of the biofilm were observed which may indicate a compromised extracellular matrix. Irradiated biofilms in the presence of gold film also showed strong propidium iodide signal which implies an increase in the number of dead bacterial cells after laser treatment. Thus, this laser-based technique is a promising approach in targeting and eradicating matured biofilms attached on surfaces such as medical implants. (Figure presented.).

KW - biofilm

KW - gold

KW - laser ablation

KW - pulsed lasers

UR - http://www.scopus.com/inward/record.url?scp=84990934149&partnerID=8YFLogxK

U2 - 10.1002/jbio.201600146

DO - 10.1002/jbio.201600146

M3 - Article

C2 - 27714933

AN - SCOPUS:84990934149

VL - 10

SP - 1043

EP - 1052

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 8

ER -