Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 62-74 |
| Seitenumfang | 13 |
| Fachzeitschrift | Biotechnology journal |
| Jahrgang | 5 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 12 Jan. 2010 |
Abstract
Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularmedizin
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in: Biotechnology journal, Jahrgang 5, Nr. 1, 12.01.2010, S. 62-74.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Statistical aspects of design and validation of microtitre-plate-based linear and non-linear parallel in vitro bioassays
AU - Zimmermann, Hanne
AU - Gerhard, Daniel
AU - Dingermann, Theo
AU - Hothorn, Ludwig A.
PY - 2010/1/12
Y1 - 2010/1/12
N2 - Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.
AB - Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.
KW - Assay validation
KW - Linear parallel-line assay
KW - Mixed model approach
KW - Non-linear parallel-line assay
KW - Variance component analysis
UR - http://www.scopus.com/inward/record.url?scp=75649098212&partnerID=8YFLogxK
U2 - 10.1002/biot.200900146
DO - 10.1002/biot.200900146
M3 - Article
C2 - 19844917
AN - SCOPUS:75649098212
VL - 5
SP - 62
EP - 74
JO - Biotechnology journal
JF - Biotechnology journal
SN - 1860-6768
IS - 1
ER -