Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 412-419 |
| Seitenumfang | 8 |
| Fachzeitschrift | Journal of biotechnology |
| Jahrgang | 124 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 20 Feb. 2006 |
Abstract
State variables throughout non-induced and induced cultivations of Pichia pastoris for the heterologous Rhizopus oryzae lipase (ROL) production were monitored with a multi-wavelength on-line fluorescence sensor. Based on this work, the use of in situ multi-wavelength fluorometry combined with chemometrics models (PLS-1 models) provided a quantitative prediction of biomass and substrates (glycerol and methanol) during non-induced and induced ROL production. The mean prediction errors for both variables were about 7% and 10%, respectively. ROL is also quite satisfactory estimated in the exponential growth phase with prediction errors similar to biomass and substrate variables. However, in the stationary phase, where proteolytic degradation of ROL is observed, the prediction error could get a value about 20%. This fact is due to the lower reproducibility of protein production from batch to batch.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Journal of biotechnology, Jahrgang 124, Nr. 2, 20.02.2006, S. 412-419.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - State variables monitoring by in situ multi-wavelength fluorescence spectroscopy in heterologous protein production by Pichia pastoris
AU - Surribas, Anna
AU - Geissler, David
AU - Gierse, Alexander
AU - Scheper, Thomas
AU - Hitzmann, Bernd
AU - Montesinos, José Luis
AU - Valero, Francisco
N1 - Funding information: A.S. is recipient of a predoctoral fellowship from the Ministerio de Educación y Ciencia (Spain). This work has been supported by the Spanish programme on Chemical Processes Technologies (CTQ2004-00300). The Department of Chemical Engineering (UAB) is member as Unit of Biochemical Engineering of the Centre de Referència en Biotecnologia de Catalunya (Generalitat de Catalunya). Authors would like to thank Martina Weiss for their contribution to this work.
PY - 2006/2/20
Y1 - 2006/2/20
N2 - State variables throughout non-induced and induced cultivations of Pichia pastoris for the heterologous Rhizopus oryzae lipase (ROL) production were monitored with a multi-wavelength on-line fluorescence sensor. Based on this work, the use of in situ multi-wavelength fluorometry combined with chemometrics models (PLS-1 models) provided a quantitative prediction of biomass and substrates (glycerol and methanol) during non-induced and induced ROL production. The mean prediction errors for both variables were about 7% and 10%, respectively. ROL is also quite satisfactory estimated in the exponential growth phase with prediction errors similar to biomass and substrate variables. However, in the stationary phase, where proteolytic degradation of ROL is observed, the prediction error could get a value about 20%. This fact is due to the lower reproducibility of protein production from batch to batch.
AB - State variables throughout non-induced and induced cultivations of Pichia pastoris for the heterologous Rhizopus oryzae lipase (ROL) production were monitored with a multi-wavelength on-line fluorescence sensor. Based on this work, the use of in situ multi-wavelength fluorometry combined with chemometrics models (PLS-1 models) provided a quantitative prediction of biomass and substrates (glycerol and methanol) during non-induced and induced ROL production. The mean prediction errors for both variables were about 7% and 10%, respectively. ROL is also quite satisfactory estimated in the exponential growth phase with prediction errors similar to biomass and substrate variables. However, in the stationary phase, where proteolytic degradation of ROL is observed, the prediction error could get a value about 20%. This fact is due to the lower reproducibility of protein production from batch to batch.
KW - Chemometrics
KW - Heterologous protein production
KW - In situ multi-wavelength fluorescence spectroscopy
KW - On-line monitoring
KW - Pichia pastoris
UR - http://www.scopus.com/inward/record.url?scp=33745190302&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2006.01.002
DO - 10.1016/j.jbiotec.2006.01.002
M3 - Article
C2 - 16488501
AN - SCOPUS:33745190302
VL - 124
SP - 412
EP - 419
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 2
ER -