TY - JOUR

T1 - SPR Biosensor Based on Polymer Multi-Mode Optical Waveguide and Nanoparticle Signal Enhancement

AU - Walter, Johanna-Gabriela

AU - Eilers, Alina

AU - Alwis, Lourdes Shanika Malindi

AU - Roth, Bernhard Wilhelm

AU - Bremer, Kort

N1 - Funding information: B.W.R. acknowledges funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453). J.-G.W. and K.B. acknowledge support from the Bundesministerium für Wirtschaft und Energie (BMWi) as well as the European Social Fund (ESF) within Grant Number 03EFHNI059. The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover. B.W.R. acknowledges funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany?s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453). J.-G.W. and K.B. acknowledge support from the Bundesministerium f?r Wirtschaft und Energie (BMWi) as well as the European Social Fund (ESF) within Grant Number 03EFHNI059. The publication of this article was funded by the Open Access Fund of Leibniz Universit?t Hannover.

PY - 2020/5/20

Y1 - 2020/5/20

N2 - We present a surface plasmon resonance (SPR) biosensor that is based on a planar-optical multi-mode (MM) polymer waveguide structure applied for the detection of biomolecules in the lower nano-molar (nM) range. The basic sensor shows a sensitivity of 608.6 nm/RIU when exposed to refractive index changes with a measurement resolution of 4.3 × 10−3 RIU. By combining the SPR sensor with an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay, the detection of C-reactive protein (CRP) in a buffer solution was achieved with a response of 0.118 nm/nM. Due to the multi-mode polymer waveguide structure and the simple concept, the reported biosensor is well suited for low-cost disposable lab-on-a-chip applications and can be used with rather simple and economic devices. In particular, the sensor offers the potential for fast and multiplexed detection of several biomarkers on a single integrated platform.

AB - We present a surface plasmon resonance (SPR) biosensor that is based on a planar-optical multi-mode (MM) polymer waveguide structure applied for the detection of biomolecules in the lower nano-molar (nM) range. The basic sensor shows a sensitivity of 608.6 nm/RIU when exposed to refractive index changes with a measurement resolution of 4.3 × 10−3 RIU. By combining the SPR sensor with an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay, the detection of C-reactive protein (CRP) in a buffer solution was achieved with a response of 0.118 nm/nM. Due to the multi-mode polymer waveguide structure and the simple concept, the reported biosensor is well suited for low-cost disposable lab-on-a-chip applications and can be used with rather simple and economic devices. In particular, the sensor offers the potential for fast and multiplexed detection of several biomarkers on a single integrated platform.

KW - Aptamer

KW - Biosensor

KW - Gold-nanoparticle

KW - Lab-on-a-chip

KW - Multi-mode

KW - Planar-optical

KW - Sensor

KW - Surface plasmon resonance (SPR)

KW - Waveguide

UR - http://www.scopus.com/inward/record.url?scp=85085241832&partnerID=8YFLogxK

U2 - 10.3390/s20102889

DO - 10.3390/s20102889

M3 - Article

C2 - 32443702

AN - SCOPUS:85085241832

VL - 20

JO - Sensors

JF - Sensors

SN - 1424-8220

IS - 10

M1 - 2889

ER -