TY - JOUR

T1 - Sour cherry strain of plum pox potyvirus (PPV)

T2 - Molecular and serological evidence for a new subgroup of PPV strains

AU - Nemchinov, L.

AU - Hadidi, A.

AU - Maiss, E.

AU - Cambra, M.

AU - Candresse, T.

AU - Damsteegt, V.

PY - 1996/11

Y1 - 1996/11

N2 - Properties of the unusual sour cherry strain of plum pox potyvirus (PPV- SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV- SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV cherry (PPV-C), and that PPV-SoC is its prototype member.

AB - Properties of the unusual sour cherry strain of plum pox potyvirus (PPV- SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV- SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV cherry (PPV-C), and that PPV-SoC is its prototype member.

KW - detection

KW - differentiation

KW - insect transmission

KW - nucleotide sequence of coat protein

KW - phylogeny

UR - http://www.scopus.com/inward/record.url?scp=0029802736&partnerID=8YFLogxK

U2 - 10.1094/Phyto-86-1215

DO - 10.1094/Phyto-86-1215

M3 - Article

AN - SCOPUS:0029802736

VL - 86

SP - 1215

EP - 1221

JO - PHYTOPATHOLOGY

JF - PHYTOPATHOLOGY

SN - 0031-949X

IS - 11

ER -