TY - JOUR

T1 - Somatic embryogenesis and transformation of the diploid Rosa chinensis cv Old Blush

AU - Vergne, Philippe

AU - Maene, Marion

AU - Gabant, Guillaume

AU - Chauvet, Aurélie

AU - Debener, Thomas

AU - Bendahmane, Mohammed

N1 - Funding Information: Acknowledgements We thank Vincent David for dedicated assistance, Isabelle Desbouchages and Alexis Lacroix for assistance in growing the plants. This work was funded by Région Rhône-Alpes (Programme ‘Essor des Biotechnologies’ 2000–2003), by the German–French collaborative program ‘Procope’ (2004–2005) and by the Plant Biology Department of the National Institute of Agronomic Research (INRA-France). MM was supported by a PhD fellowship ‘Prospective’ from Région Rhône-Alpes (2005–2008).

PY - 2010/1

Y1 - 2010/1

N2 - Somatic embryogenesis was induced from in vitro-derived leaf explants of Rosa chinensis cultivar (cv) Old Blush. Calli producing embryos with expanded cotyledons (RcOBType1 embryos) were obtained. Further refinements of the callus maintenance medium generated a more typical rose embryogenic callus (RcOBType2) displaying high levels of secondary embryogenesis and embryos with limited cotyledon expansion Agrobacteriumtumefaciens-mediated transformation assays using β-glucuronidase (GUS) reporter gene showed that both types of embryos were competent for transformation. Under selection conditions, transformed RcOBType1 explants produced non chimaeric transformed embryos, from which shoots could be adventitiously regenerated. In contrast to RcOBType1, transformed RcOBType2 embryos directly yielded transformed shoots when repeatedly cultured in selective regeneration conditions. Transformation efficiency ranged between three to nine percent and shoots suitable for rooting were obtained within 6-8 months. Transgenic plants were transferred into the greenhouse and molecularly confirmed. The availability of transformation methods in a diploid rose, R. chinensis cv. Old Blush, will be useful for gene functional studies.

AB - Somatic embryogenesis was induced from in vitro-derived leaf explants of Rosa chinensis cultivar (cv) Old Blush. Calli producing embryos with expanded cotyledons (RcOBType1 embryos) were obtained. Further refinements of the callus maintenance medium generated a more typical rose embryogenic callus (RcOBType2) displaying high levels of secondary embryogenesis and embryos with limited cotyledon expansion Agrobacteriumtumefaciens-mediated transformation assays using β-glucuronidase (GUS) reporter gene showed that both types of embryos were competent for transformation. Under selection conditions, transformed RcOBType1 explants produced non chimaeric transformed embryos, from which shoots could be adventitiously regenerated. In contrast to RcOBType1, transformed RcOBType2 embryos directly yielded transformed shoots when repeatedly cultured in selective regeneration conditions. Transformation efficiency ranged between three to nine percent and shoots suitable for rooting were obtained within 6-8 months. Transgenic plants were transferred into the greenhouse and molecularly confirmed. The availability of transformation methods in a diploid rose, R. chinensis cv. Old Blush, will be useful for gene functional studies.

KW - Diploid

KW - Genetic transformation

KW - Somatic embryogenesis

UR - http://www.scopus.com/inward/record.url?scp=72249087787&partnerID=8YFLogxK

U2 - 10.1007/s11240-009-9621-z

DO - 10.1007/s11240-009-9621-z

M3 - Article

AN - SCOPUS:72249087787

VL - 100

SP - 73

EP - 81

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -