Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 71-79 |
| Seitenumfang | 9 |
| Fachzeitschrift | Soil Biology and Biochemistry |
| Jahrgang | 113 |
| Publikationsstatus | Veröffentlicht - Okt. 2017 |
Abstract
Many soil functions are modulated by processes at soil biogeochemical interfaces (BGIs). However, characterizing the elemental dynamics at BGIs is hampered by the heterogeneity of soil microenvironments. In order to investigate the processes of BGI formation in an upland soil (Mollisol) and a paddy soil (Oxisol), we developed a SoilChip method by assembling dispersed soil particles onto homogeneous 800-μm-diameter microarray chips and then submerging them in a solution that contained dissolved organic matter (OM) extracted from one of the two soils. The chips with Mollisol particles were incubated at 95–100% humidity, whereas the chips with Oxisol particles were incubated at 100% humidity. Dynamics of individual elements at the soils’ BGIs were quantitatively determined using X-ray photoelectron spectroscopy (XPS). Distinct differences in the soil-microbe complexes and elemental dynamics between the Mollisol and Oxisol BGIs suggested that the formation of specific BGIs resulted from the complex interaction of physical, chemical, and microbial processes. By integrating the SoilChip and XPS, it was possible to elucidate the dynamic formation of the two different soil BGIs under standardized conditions. Therefore, the SoilChip method is a promising tool for investigating micro-ecological processes in soil.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Mikrobiologie
- Agrar- und Biowissenschaften (insg.)
- Bodenkunde
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Soil Biology and Biochemistry, Jahrgang 113, 10.2017, S. 71-79.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - SoilChip-XPS integrated technique to study formation of soil biogeochemical interfaces
AU - Huang, Xizhi
AU - Li, Yiwei
AU - Liu, Bifeng
AU - Guggenberger, Georg
AU - Shibistova, Olga
AU - Zhu, Zhenke
AU - Ge, Tida
AU - Tan, Wenfeng
AU - Wu, Jinshui
N1 - Funding information: This work was funded by the National Natural Science Foundation of China (Grant No. 41522107; 41430860), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB15020401), Youth Innovation Team Project of ISA, CAS (2017QNCXTD_GTD) and the Recruitment Program of High-End Foreign Experts of the State Administration of Foreign Experts Affairs (GDT20154300073, awarded to Georg Guggenberger). We are also grateful to Xixian Wang, Anle Ge and Yachao Wang for their help on the soft lithography methods, and to Zhang Zhang, Shuangqian Yan and Xufu Xiang for their help on the characterization of the particle size distribution, and SEM-EDX analysis, respectively.
PY - 2017/10
Y1 - 2017/10
N2 - Many soil functions are modulated by processes at soil biogeochemical interfaces (BGIs). However, characterizing the elemental dynamics at BGIs is hampered by the heterogeneity of soil microenvironments. In order to investigate the processes of BGI formation in an upland soil (Mollisol) and a paddy soil (Oxisol), we developed a SoilChip method by assembling dispersed soil particles onto homogeneous 800-μm-diameter microarray chips and then submerging them in a solution that contained dissolved organic matter (OM) extracted from one of the two soils. The chips with Mollisol particles were incubated at 95–100% humidity, whereas the chips with Oxisol particles were incubated at 100% humidity. Dynamics of individual elements at the soils’ BGIs were quantitatively determined using X-ray photoelectron spectroscopy (XPS). Distinct differences in the soil-microbe complexes and elemental dynamics between the Mollisol and Oxisol BGIs suggested that the formation of specific BGIs resulted from the complex interaction of physical, chemical, and microbial processes. By integrating the SoilChip and XPS, it was possible to elucidate the dynamic formation of the two different soil BGIs under standardized conditions. Therefore, the SoilChip method is a promising tool for investigating micro-ecological processes in soil.
AB - Many soil functions are modulated by processes at soil biogeochemical interfaces (BGIs). However, characterizing the elemental dynamics at BGIs is hampered by the heterogeneity of soil microenvironments. In order to investigate the processes of BGI formation in an upland soil (Mollisol) and a paddy soil (Oxisol), we developed a SoilChip method by assembling dispersed soil particles onto homogeneous 800-μm-diameter microarray chips and then submerging them in a solution that contained dissolved organic matter (OM) extracted from one of the two soils. The chips with Mollisol particles were incubated at 95–100% humidity, whereas the chips with Oxisol particles were incubated at 100% humidity. Dynamics of individual elements at the soils’ BGIs were quantitatively determined using X-ray photoelectron spectroscopy (XPS). Distinct differences in the soil-microbe complexes and elemental dynamics between the Mollisol and Oxisol BGIs suggested that the formation of specific BGIs resulted from the complex interaction of physical, chemical, and microbial processes. By integrating the SoilChip and XPS, it was possible to elucidate the dynamic formation of the two different soil BGIs under standardized conditions. Therefore, the SoilChip method is a promising tool for investigating micro-ecological processes in soil.
KW - Lab on a chip
KW - Mollisol
KW - Oxisol
KW - Soil biogeochemical interface
KW - SoilChip
KW - X-ray photoelectron spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85020278921&partnerID=8YFLogxK
U2 - 10.1016/j.soilbio.2017.05.021
DO - 10.1016/j.soilbio.2017.05.021
M3 - Article
AN - SCOPUS:85020278921
VL - 113
SP - 71
EP - 79
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
ER -