TY - JOUR

T1 - Smart sustainable bottle (SSB) system for E. coli based recombinant protein production

AU - Li, Zhaopeng

AU - Carstensen, Bettina

AU - Rinas, Ursula

N1 - Funding Information: Partial financial support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) for the Cluster of Excellence REBIRTH is gratefully acknowledged.

PY - 2014/11/5

Y1 - 2014/11/5

N2 - Background: Recombinant proteins are usually required in laboratories interested in the protein but not in the production process itself. Thus, technical equipment which is easy to handle and straight forward protein production procedures are of great benefit to those laboratories. Companies selling single use cultivation bags and bioreactors are trying to satisfy at least part of these needs. However, single-use systems can contribute to major costs which might be acceptable when "good manufacturing practices" are required but not acceptable for most laboratories facing tight funding. Results: The assembly and application of a simple self-made "smart sustainable bottle" (SSB) system for E. coli based protein production is presented. The core of the SSB system is a 2-L glass bottle which is operated at constant temperature, air flow, and stirrer speed without measurement and control of pH and dissolved oxygen. Oxygen transfer capacities are in the range as in conventional bioreactors operated at intermediate aeration rates and by far exceed those found in conventional shaking flasks and disposable bioreactors. The SSB system was applied for the production of various recombinant proteins using T7-based expression systems and a defined autoinduction medium. The production performance regarding amount and solubility of proteins with robust and delicate properties was as good as in state-of-the-art stirred tank commercial bioreactors. Conclusions: The SSB system represents a low cost protein production device applicable for easy, effective, and reproducible recombinant protein production.

AB - Background: Recombinant proteins are usually required in laboratories interested in the protein but not in the production process itself. Thus, technical equipment which is easy to handle and straight forward protein production procedures are of great benefit to those laboratories. Companies selling single use cultivation bags and bioreactors are trying to satisfy at least part of these needs. However, single-use systems can contribute to major costs which might be acceptable when "good manufacturing practices" are required but not acceptable for most laboratories facing tight funding. Results: The assembly and application of a simple self-made "smart sustainable bottle" (SSB) system for E. coli based protein production is presented. The core of the SSB system is a 2-L glass bottle which is operated at constant temperature, air flow, and stirrer speed without measurement and control of pH and dissolved oxygen. Oxygen transfer capacities are in the range as in conventional bioreactors operated at intermediate aeration rates and by far exceed those found in conventional shaking flasks and disposable bioreactors. The SSB system was applied for the production of various recombinant proteins using T7-based expression systems and a defined autoinduction medium. The production performance regarding amount and solubility of proteins with robust and delicate properties was as good as in state-of-the-art stirred tank commercial bioreactors. Conclusions: The SSB system represents a low cost protein production device applicable for easy, effective, and reproducible recombinant protein production.

KW - Autoinduction

KW - Escherichia coli

KW - ka

KW - Oxygen transfer

KW - Self-made bioreactor

UR - http://www.scopus.com/inward/record.url?scp=84920759089&partnerID=8YFLogxK

U2 - 10.1186/s12934-014-0153-9

DO - 10.1186/s12934-014-0153-9

M3 - Article

C2 - 25369866

AN - SCOPUS:84920759089

VL - 13

JO - Microbial cell factories

JF - Microbial cell factories

SN - 1475-2859

IS - 1

M1 - 153

ER -