TY - JOUR

T1 - Single-run analysis of vitamin D photoproducts in oyster mushroom (Pleurotus ostreatus) after UV-B treatment

AU - Wittig, Maximilian

AU - Krings, Ulrich

AU - Berger, Ralf G.

N1 - Funding information: The authors wish to express their thanks to Martin Rühl, Druid Austernpilze, for providing fresh and certified mushroom material. This project was supported by the Lower Saxony Ministry of Science and Arts , FAEN project #6.

PY - 2013/8/30

Y1 - 2013/8/30

N2 - A single-run high performance liquid chromatography (HPLC) with diode array detector (DAD) based method was developed for the separation, identification and comprehensive quantification of degradation products of ergosterol formed in oyster mushroom (Pleurotus ostreatus) after UV-B exposure. After 60min, 10 substances involved in the photoprocess were separated, identified by their characteristic DAD spectrum and distinguished by their molecular weight, in cases where spectra were identical: vitamin D2, previtamin D2, tachysterol2, lumisterol2 and ergosterol, and, in minor quantity, their structural analogues of the 22,23-dihydroergocalciferol (vitamin D4) series. Sample preparation protocol affected the total yields and the ratios of previtamin and vitamin D2/D4. Hot alkaline hydrolysis resulted in the best digestion of the mushroom matrix and accordingly gave the highest vitamin D yield (D2: 141.32μgg-1 dry matter, DM; D4: 22.72μgg-1 DM). Limit of detection for vitamin D2/4 was 0.02μgg-1 dry matter (DM) and was estimated for previtamin D2/4 (0.06μgg-1 DM), tachysterol2/4 (0.02μgg-1 DM) and lumisterol2/4 (0.06μgg-1 DM). Recovery of spiked vitamin D2 was 97±0.7%. The study provides an analytical tool to assess the process of vitamin D generation after UV-B treatment for the production of oyster mushrooms with a balanced nutritional profile of vitamin D compounds.

AB - A single-run high performance liquid chromatography (HPLC) with diode array detector (DAD) based method was developed for the separation, identification and comprehensive quantification of degradation products of ergosterol formed in oyster mushroom (Pleurotus ostreatus) after UV-B exposure. After 60min, 10 substances involved in the photoprocess were separated, identified by their characteristic DAD spectrum and distinguished by their molecular weight, in cases where spectra were identical: vitamin D2, previtamin D2, tachysterol2, lumisterol2 and ergosterol, and, in minor quantity, their structural analogues of the 22,23-dihydroergocalciferol (vitamin D4) series. Sample preparation protocol affected the total yields and the ratios of previtamin and vitamin D2/D4. Hot alkaline hydrolysis resulted in the best digestion of the mushroom matrix and accordingly gave the highest vitamin D yield (D2: 141.32μgg-1 dry matter, DM; D4: 22.72μgg-1 DM). Limit of detection for vitamin D2/4 was 0.02μgg-1 dry matter (DM) and was estimated for previtamin D2/4 (0.06μgg-1 DM), tachysterol2/4 (0.02μgg-1 DM) and lumisterol2/4 (0.06μgg-1 DM). Recovery of spiked vitamin D2 was 97±0.7%. The study provides an analytical tool to assess the process of vitamin D generation after UV-B treatment for the production of oyster mushrooms with a balanced nutritional profile of vitamin D compounds.

KW - 22,23-Dihydroergocalciferol

KW - Ergocalciferol (vitamin D)

KW - Food analysis

KW - Food composition

KW - Lumisterol

KW - Photoisomers

KW - Pleurotus ostreatus

KW - Previtamin D

KW - Tachysterol

KW - Vitamin D activity

KW - Vitamin D

UR - http://www.scopus.com/inward/record.url?scp=84886239093&partnerID=8YFLogxK

U2 - 10.1016/j.jfca.2013.05.017

DO - 10.1016/j.jfca.2013.05.017

M3 - Article

AN - SCOPUS:84886239093

VL - 31

SP - 266

EP - 274

JO - Journal of Food Composition and Analysis

JF - Journal of Food Composition and Analysis

SN - 0889-1575

IS - 2

ER -