Setup of a fiber optical oxygen multisensor-system and its applications in biotechnology

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

Organisationseinheiten

Externe Organisationen

  • Max-Planck-Institut für Marine Mikrobiologie
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)121-130
Seitenumfang10
FachzeitschriftSensors and Actuators, B: Chemical
Jahrgang70
Ausgabenummer1-3
PublikationsstatusVeröffentlicht - 16 Okt. 2000

Abstract

The development of a simple and very easy-to-use fiber optical oxygen multisensor measuring system is described. This fiber optical chemosensor (optode) is based on the fluorescence quenching of a special fluorophor by oxygen. Standard applications of the well-established amperometric oxygen sensor can be carried out with this optical sensor. Beyond that, many special applications for oxygen measurement become possible (O.S. Wolfbeis, Fiber Optic Chemical Sensors and Biosensors, vols. I and II, CRC Press, Boca Raton, USA, 1991), which cannot be performed by sensors of the Clark (L.C. Clark, Electrochemical device for chemical analysis, US Patent 2913386, 1956) type. In comparison to electrochemical sensors fiber optical chemosensors have several advantages, which are illustrated in this paper. Details of the sensor chemistry, instrumental system and the oxygen sensitivity are presented. The application in a precultivation processes, in Ca-alginate beads and layers with immobilized cells in packed-bed-reactors are presented. Diffusion coefficients were calculated from the experiments.

ASJC Scopus Sachgebiete

Zitieren

Setup of a fiber optical oxygen multisensor-system and its applications in biotechnology. / Kohls, O.; Scheper, Th.
in: Sensors and Actuators, B: Chemical, Jahrgang 70, Nr. 1-3, 16.10.2000, S. 121-130.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Download
@article{71a4c23b50074ba18be82ab825bca0e7,
title = "Setup of a fiber optical oxygen multisensor-system and its applications in biotechnology",
abstract = "The development of a simple and very easy-to-use fiber optical oxygen multisensor measuring system is described. This fiber optical chemosensor (optode) is based on the fluorescence quenching of a special fluorophor by oxygen. Standard applications of the well-established amperometric oxygen sensor can be carried out with this optical sensor. Beyond that, many special applications for oxygen measurement become possible (O.S. Wolfbeis, Fiber Optic Chemical Sensors and Biosensors, vols. I and II, CRC Press, Boca Raton, USA, 1991), which cannot be performed by sensors of the Clark (L.C. Clark, Electrochemical device for chemical analysis, US Patent 2913386, 1956) type. In comparison to electrochemical sensors fiber optical chemosensors have several advantages, which are illustrated in this paper. Details of the sensor chemistry, instrumental system and the oxygen sensitivity are presented. The application in a precultivation processes, in Ca-alginate beads and layers with immobilized cells in packed-bed-reactors are presented. Diffusion coefficients were calculated from the experiments.",
author = "O. Kohls and Th Scheper",
year = "2000",
month = oct,
day = "16",
doi = "10.1016/S0925-4005(00)00581-5",
language = "English",
volume = "70",
pages = "121--130",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "1-3",

}

Download

TY - JOUR

T1 - Setup of a fiber optical oxygen multisensor-system and its applications in biotechnology

AU - Kohls, O.

AU - Scheper, Th

PY - 2000/10/16

Y1 - 2000/10/16

N2 - The development of a simple and very easy-to-use fiber optical oxygen multisensor measuring system is described. This fiber optical chemosensor (optode) is based on the fluorescence quenching of a special fluorophor by oxygen. Standard applications of the well-established amperometric oxygen sensor can be carried out with this optical sensor. Beyond that, many special applications for oxygen measurement become possible (O.S. Wolfbeis, Fiber Optic Chemical Sensors and Biosensors, vols. I and II, CRC Press, Boca Raton, USA, 1991), which cannot be performed by sensors of the Clark (L.C. Clark, Electrochemical device for chemical analysis, US Patent 2913386, 1956) type. In comparison to electrochemical sensors fiber optical chemosensors have several advantages, which are illustrated in this paper. Details of the sensor chemistry, instrumental system and the oxygen sensitivity are presented. The application in a precultivation processes, in Ca-alginate beads and layers with immobilized cells in packed-bed-reactors are presented. Diffusion coefficients were calculated from the experiments.

AB - The development of a simple and very easy-to-use fiber optical oxygen multisensor measuring system is described. This fiber optical chemosensor (optode) is based on the fluorescence quenching of a special fluorophor by oxygen. Standard applications of the well-established amperometric oxygen sensor can be carried out with this optical sensor. Beyond that, many special applications for oxygen measurement become possible (O.S. Wolfbeis, Fiber Optic Chemical Sensors and Biosensors, vols. I and II, CRC Press, Boca Raton, USA, 1991), which cannot be performed by sensors of the Clark (L.C. Clark, Electrochemical device for chemical analysis, US Patent 2913386, 1956) type. In comparison to electrochemical sensors fiber optical chemosensors have several advantages, which are illustrated in this paper. Details of the sensor chemistry, instrumental system and the oxygen sensitivity are presented. The application in a precultivation processes, in Ca-alginate beads and layers with immobilized cells in packed-bed-reactors are presented. Diffusion coefficients were calculated from the experiments.

UR - http://www.scopus.com/inward/record.url?scp=0034320861&partnerID=8YFLogxK

U2 - 10.1016/S0925-4005(00)00581-5

DO - 10.1016/S0925-4005(00)00581-5

M3 - Article

AN - SCOPUS:0034320861

VL - 70

SP - 121

EP - 130

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 1-3

ER -

Von denselben Autoren