Details
Originalsprache | Englisch |
---|---|
Aufsatznummer | fiab031 |
Fachzeitschrift | FEMS microbiology ecology |
Jahrgang | 97 |
Ausgabenummer | 4 |
Publikationsstatus | Veröffentlicht - Apr. 2021 |
Abstract
A soil column split-root experiment was designed to investigate the ability of apple replant disease (ARD)-causing agents to spread in soil. 'M26' apple rootstocks grew into a top layer of Control soil, followed by a barrier-free split-soil layer (Control soil/ARD soil). We observed a severely reduced root growth, concomitant with enhanced gene expression of phytoalexin biosynthetic genes and phytoalexin content in roots from ARD soil, indicating a pronounced local plant defense response. Amplicon sequencing (bacteria, archaea, fungi) revealed local shifts in diversity and composition of microorganisms in the rhizoplane of roots from ARD soil. An enrichment of operational taxonomic units affiliated to potential ARD fungal pathogens (Ilyonectria and Nectria sp.) and bacteria frequently associated with ARD (Streptomyces, Variovorax) was noted. In conclusion, our integrated study supports the idea of ARD being local and not spreading into surrounding soil, as only the roots in ARD soil were affected in terms of growth, phytoalexin biosynthetic gene expression, phytoalexin production and altered microbiome structure. This study further reinforces the microbiological nature of ARD, being likely triggered by a disturbed soil microbiome enriched with low mobility of the ARD-causing agents that induce a strong plant defense and rhizoplane microbiome dysbiosis, concurring with root damage.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Mikrobiologie
- Umweltwissenschaften (insg.)
- Ökologie
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: FEMS microbiology ecology, Jahrgang 97, Nr. 4, fiab031, 04.2021.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Root exposure to apple replant disease soil triggers local defense response and rhizoplane microbiome dysbiosis
AU - Balbín-Suárez, Alicia
AU - Jacquiod, Samuel
AU - Rohr, Annmarie Deetja
AU - Liu, Benye
AU - Flachowsky, Henryk
AU - Winkelmann, Traud
AU - Beerhues, Ludger
AU - Nesme, Joseph
AU - Sørensen, Søren J.
AU - Vetterlein, Doris
AU - Smalla, Kornelia
N1 - We thank Andreas Wrede and the UFZ Workshop for kindly providing us with the material necessary to conduct the experiment (field site maintenance and split-root columns, respectively). We thank Ina-Maria Zickenrott for conducting the column experiment, X-ray CT scanning and all WinRHIZO analyses. We thank Maik Lucas for time resolved X-ray CT analyses and Eva Lippold for calculation of X-ray CT dose. We thank Jasmin Schmidt for excellent technical assistance, and Doreen Babin and DavidAlain Barbeoc’h for great scientific and graphic design advice, ´ respectively This work was supported by the BonaRes initiative of the German Federal Ministry of Education and Research (BMBF, Bundesministerium fur Bildung und Forschung) (FZK 031B0025G, ¨ FZK 031B0025A and FZK 031B0025B). Samuel Jacquiod was supported by the Universite de Bourgogne Franche-Comt ´ e via ´ the ISITE-BFC International Junior Fellowship award (AAP3: RA19028.AEC.IS). The work of Annmarie-Deetja Rohr was part of the Research Training Group GRK1798 ‘Signaling at the Plant– Soil Interface’ funded by the German Research Foundation (DFG, Deutsche Forschungsgemeinschaft).
PY - 2021/4
Y1 - 2021/4
N2 - A soil column split-root experiment was designed to investigate the ability of apple replant disease (ARD)-causing agents to spread in soil. 'M26' apple rootstocks grew into a top layer of Control soil, followed by a barrier-free split-soil layer (Control soil/ARD soil). We observed a severely reduced root growth, concomitant with enhanced gene expression of phytoalexin biosynthetic genes and phytoalexin content in roots from ARD soil, indicating a pronounced local plant defense response. Amplicon sequencing (bacteria, archaea, fungi) revealed local shifts in diversity and composition of microorganisms in the rhizoplane of roots from ARD soil. An enrichment of operational taxonomic units affiliated to potential ARD fungal pathogens (Ilyonectria and Nectria sp.) and bacteria frequently associated with ARD (Streptomyces, Variovorax) was noted. In conclusion, our integrated study supports the idea of ARD being local and not spreading into surrounding soil, as only the roots in ARD soil were affected in terms of growth, phytoalexin biosynthetic gene expression, phytoalexin production and altered microbiome structure. This study further reinforces the microbiological nature of ARD, being likely triggered by a disturbed soil microbiome enriched with low mobility of the ARD-causing agents that induce a strong plant defense and rhizoplane microbiome dysbiosis, concurring with root damage.
AB - A soil column split-root experiment was designed to investigate the ability of apple replant disease (ARD)-causing agents to spread in soil. 'M26' apple rootstocks grew into a top layer of Control soil, followed by a barrier-free split-soil layer (Control soil/ARD soil). We observed a severely reduced root growth, concomitant with enhanced gene expression of phytoalexin biosynthetic genes and phytoalexin content in roots from ARD soil, indicating a pronounced local plant defense response. Amplicon sequencing (bacteria, archaea, fungi) revealed local shifts in diversity and composition of microorganisms in the rhizoplane of roots from ARD soil. An enrichment of operational taxonomic units affiliated to potential ARD fungal pathogens (Ilyonectria and Nectria sp.) and bacteria frequently associated with ARD (Streptomyces, Variovorax) was noted. In conclusion, our integrated study supports the idea of ARD being local and not spreading into surrounding soil, as only the roots in ARD soil were affected in terms of growth, phytoalexin biosynthetic gene expression, phytoalexin production and altered microbiome structure. This study further reinforces the microbiological nature of ARD, being likely triggered by a disturbed soil microbiome enriched with low mobility of the ARD-causing agents that induce a strong plant defense and rhizoplane microbiome dysbiosis, concurring with root damage.
KW - phytoalexins
KW - root system architecture
KW - soil microbiome
KW - split-root experiment
KW - X-ray computed tomography
UR - http://www.scopus.com/inward/record.url?scp=85103683308&partnerID=8YFLogxK
U2 - 10.1093/femsec/fiab031
DO - 10.1093/femsec/fiab031
M3 - Article
C2 - 33587112
AN - SCOPUS:85103683308
VL - 97
JO - FEMS microbiology ecology
JF - FEMS microbiology ecology
SN - 0168-6496
IS - 4
M1 - fiab031
ER -