Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1758-1762 |
| Seitenumfang | 5 |
| Fachzeitschrift | Journal of the American Chemical Society |
| Jahrgang | 143 |
| Ausgabenummer | 4 |
| Frühes Online-Datum | 20 Jan. 2021 |
| Publikationsstatus | Veröffentlicht - 3 Feb. 2021 |
Abstract
Biomineralization occurs in aqueous environments. Despite the ubiquity and relevance of CaCO3 biomineralization, the role of water in the biomineralization process has remained elusive. Here, we demonstrate that water reorganization accompanies CaCO3 biomineralization for sea urchin spine generation in a model system. Using surface-specific vibrational spectroscopy, we probe the water at the interface of the spine-associated protein during CaCO3 mineralization. Our results show that, while the protein structure remains unchanged, the structure of interfacial water is perturbed differently in the presence of both Ca2+ and CO32- compared to the addition of only Ca2+. This difference is attributed to the condensation of prenucleation mineral species. Our findings are consistent with a nonclassical mineralization pathway for sea urchin spine generation and highlight the importance of protein hydration in biomineralization.
ASJC Scopus Sachgebiete
- Chemische Verfahrenstechnik (insg.)
- Katalyse
- Chemie (insg.)
- Allgemeine Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Chemische Verfahrenstechnik (insg.)
- Kolloid- und Oberflächenchemie
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in: Journal of the American Chemical Society, Jahrgang 143, Nr. 4, 03.02.2021, S. 1758-1762.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Role of Water in CaCO3 Biomineralization
AU - Lu, Hao
AU - Huang, Yu-Chieh
AU - Hunger, Johannes
AU - Gebauer, Denis
AU - Cölfen, Helmut
AU - Bonn, Mischa
N1 - Funding Information: We acknowledge financial support from Max Planck Society and the MaxWater Initiative of the Max Planck Society.
PY - 2021/2/3
Y1 - 2021/2/3
N2 - Biomineralization occurs in aqueous environments. Despite the ubiquity and relevance of CaCO3 biomineralization, the role of water in the biomineralization process has remained elusive. Here, we demonstrate that water reorganization accompanies CaCO3 biomineralization for sea urchin spine generation in a model system. Using surface-specific vibrational spectroscopy, we probe the water at the interface of the spine-associated protein during CaCO3 mineralization. Our results show that, while the protein structure remains unchanged, the structure of interfacial water is perturbed differently in the presence of both Ca2+ and CO32- compared to the addition of only Ca2+. This difference is attributed to the condensation of prenucleation mineral species. Our findings are consistent with a nonclassical mineralization pathway for sea urchin spine generation and highlight the importance of protein hydration in biomineralization.
AB - Biomineralization occurs in aqueous environments. Despite the ubiquity and relevance of CaCO3 biomineralization, the role of water in the biomineralization process has remained elusive. Here, we demonstrate that water reorganization accompanies CaCO3 biomineralization for sea urchin spine generation in a model system. Using surface-specific vibrational spectroscopy, we probe the water at the interface of the spine-associated protein during CaCO3 mineralization. Our results show that, while the protein structure remains unchanged, the structure of interfacial water is perturbed differently in the presence of both Ca2+ and CO32- compared to the addition of only Ca2+. This difference is attributed to the condensation of prenucleation mineral species. Our findings are consistent with a nonclassical mineralization pathway for sea urchin spine generation and highlight the importance of protein hydration in biomineralization.
UR - http://www.scopus.com/inward/record.url?scp=85100205720&partnerID=8YFLogxK
U2 - 10.1021/jacs.0c11976
DO - 10.1021/jacs.0c11976
M3 - Article
VL - 143
SP - 1758
EP - 1762
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 4
ER -