Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1223-1230 |
| Seitenumfang | 8 |
| Fachzeitschrift | Pakistan Journal of Pharmaceutical Sciences |
| Jahrgang | 29 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - Juli 2016 |
| Extern publiziert | Ja |
Abstract
The present study investigates the effect of different growth media and chemical enhancer on silent genes in Aspergillus carbonarius (NRL-369) for secondary metabolites production and its in vitro biological activities. Results revealed that Aspergillus carbonarius (NRL-369) grown in Czapeak yeast extract broth medium produced more metabolites compared with other media. Chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) at concentration of 15mM were effective for the expression of silent genes resulting in increased secondary metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane showed variable degree of growth inhibitions of the tested microorganisms. Similarly, these samples were also active against brine shrimps and Lemna.
ASJC Scopus Sachgebiete
- Pharmakologie, Toxikologie und Pharmazie (insg.)
- Pharmazeutische Wissenschaften
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in: Pakistan Journal of Pharmaceutical Sciences, Jahrgang 29, Nr. 4, 07.2016, S. 1223-1230.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Role of growth media and chemical enhancers in secondary metabolites production from Aspergillus carbonarius (NRL-369) and their pharmaceutical potentials
AU - Khan, Abid Ali
AU - Bacha, Nafess
AU - Ahmad, Bashir
AU - Cox, R. J.
AU - Bakht, Jehan
PY - 2016/7
Y1 - 2016/7
N2 - The present study investigates the effect of different growth media and chemical enhancer on silent genes in Aspergillus carbonarius (NRL-369) for secondary metabolites production and its in vitro biological activities. Results revealed that Aspergillus carbonarius (NRL-369) grown in Czapeak yeast extract broth medium produced more metabolites compared with other media. Chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) at concentration of 15mM were effective for the expression of silent genes resulting in increased secondary metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane showed variable degree of growth inhibitions of the tested microorganisms. Similarly, these samples were also active against brine shrimps and Lemna.
AB - The present study investigates the effect of different growth media and chemical enhancer on silent genes in Aspergillus carbonarius (NRL-369) for secondary metabolites production and its in vitro biological activities. Results revealed that Aspergillus carbonarius (NRL-369) grown in Czapeak yeast extract broth medium produced more metabolites compared with other media. Chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) at concentration of 15mM were effective for the expression of silent genes resulting in increased secondary metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane showed variable degree of growth inhibitions of the tested microorganisms. Similarly, these samples were also active against brine shrimps and Lemna.
KW - Aspergillus carbonarius
KW - Chemical enhancers
KW - Growth media
KW - Pharmaceutical activities
KW - Silient genes
UR - http://www.scopus.com/inward/record.url?scp=84979659045&partnerID=8YFLogxK
M3 - Article
C2 - 27393435
AN - SCOPUS:84979659045
VL - 29
SP - 1223
EP - 1230
JO - Pakistan Journal of Pharmaceutical Sciences
JF - Pakistan Journal of Pharmaceutical Sciences
SN - 1011-601X
IS - 4
ER -