Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Piera De Santis
  • Deborah Wegstein
  • Bastien O. Burek
  • Jacqueline Patzsch
  • Miguel Alcalde
  • Wolfgang Kroutil
  • Jonathan Z. Bloh
  • Selin Kara

Organisationseinheiten

Externe Organisationen

  • Aarhus University
  • DECHEMA-Forschungsinstitut (DFI)
  • Spanish National Research Council (CSIC)
  • Universität Graz
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Details

OriginalspracheEnglisch
Aufsatznummere202300613
FachzeitschriftCHEMSUSCHEM
Jahrgang16
Ausgabenummer23
PublikationsstatusVeröffentlicht - 7 Dez. 2023

Abstract

Unspecific peroxygenases have attracted interest in synthetic chemistry, especially for the oxidative activation of C−H bonds, as they only require hydrogen peroxide (H2O2) instead of a cofactor. Due to their instability in even small amounts of H2O2, different strategies like enzyme immobilization or in situ H2O2 production have been developed to improve the stability of these enzymes. While most strategies have been studied separately, a combination of photocatalysis with immobilized enzymes was only recently reported. To show the advantages and limiting factors of immobilized enzyme in a photobiocatalytic reaction, a comparison is made between free and immobilized enzymes. Adjustment of critical parameters such as (i) enzyme and substrate concentration, (ii) illumination wavelength and (iii) light intensity results in significantly increased enzyme stabilities of the immobilized variant. Moreover, under optimized conditions a turnover number of 334,500 was reached.

Zitieren

Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase. / De Santis, Piera; Wegstein, Deborah; Burek, Bastien O. et al.
in: CHEMSUSCHEM, Jahrgang 16, Nr. 23, e202300613, 07.12.2023.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

De Santis, P, Wegstein, D, Burek, BO, Patzsch, J, Alcalde, M, Kroutil, W, Bloh, JZ & Kara, S 2023, 'Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase', CHEMSUSCHEM, Jg. 16, Nr. 23, e202300613. https://doi.org/10.1002/cssc.202300613
De Santis, P., Wegstein, D., Burek, B. O., Patzsch, J., Alcalde, M., Kroutil, W., Bloh, J. Z., & Kara, S. (2023). Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase. CHEMSUSCHEM, 16(23), Artikel e202300613. https://doi.org/10.1002/cssc.202300613
De Santis P, Wegstein D, Burek BO, Patzsch J, Alcalde M, Kroutil W et al. Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase. CHEMSUSCHEM. 2023 Dez 7;16(23):e202300613. doi: 10.1002/cssc.202300613
De Santis, Piera ; Wegstein, Deborah ; Burek, Bastien O. et al. / Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase. in: CHEMSUSCHEM. 2023 ; Jahrgang 16, Nr. 23.
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title = "Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase",
abstract = "Unspecific peroxygenases have attracted interest in synthetic chemistry, especially for the oxidative activation of C−H bonds, as they only require hydrogen peroxide (H2O2) instead of a cofactor. Due to their instability in even small amounts of H2O2, different strategies like enzyme immobilization or in situ H2O2 production have been developed to improve the stability of these enzymes. While most strategies have been studied separately, a combination of photocatalysis with immobilized enzymes was only recently reported. To show the advantages and limiting factors of immobilized enzyme in a photobiocatalytic reaction, a comparison is made between free and immobilized enzymes. Adjustment of critical parameters such as (i) enzyme and substrate concentration, (ii) illumination wavelength and (iii) light intensity results in significantly increased enzyme stabilities of the immobilized variant. Moreover, under optimized conditions a turnover number of 334,500 was reached.",
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AU - De Santis, Piera

AU - Wegstein, Deborah

AU - Burek, Bastien O.

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AU - Alcalde, Miguel

AU - Kroutil, Wolfgang

AU - Bloh, Jonathan Z.

AU - Kara, Selin

N1 - Funding Information: f . S.K. acknowledges Independent Research Fund Denmark (PHOTOX‐ project, grant No 9063‐00031B) for the grant funding in the framework of Sapere Aude DFF Starting Grant. This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska‐Curie grant agreement No 764920. Deutsche Forschungsgemeinschaft (DFG) under funding code BL 1425/1‐2 is gratefully acknowledged

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N2 - Unspecific peroxygenases have attracted interest in synthetic chemistry, especially for the oxidative activation of C−H bonds, as they only require hydrogen peroxide (H2O2) instead of a cofactor. Due to their instability in even small amounts of H2O2, different strategies like enzyme immobilization or in situ H2O2 production have been developed to improve the stability of these enzymes. While most strategies have been studied separately, a combination of photocatalysis with immobilized enzymes was only recently reported. To show the advantages and limiting factors of immobilized enzyme in a photobiocatalytic reaction, a comparison is made between free and immobilized enzymes. Adjustment of critical parameters such as (i) enzyme and substrate concentration, (ii) illumination wavelength and (iii) light intensity results in significantly increased enzyme stabilities of the immobilized variant. Moreover, under optimized conditions a turnover number of 334,500 was reached.

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