Rhizosphere microbial communities associated to rose replant disease: links to plant growth and root metabolites

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • B. Yim
  • A. Baumann
  • Gisela Grunewaldt-Stöcker
  • B. Liu
  • L. Beerhues
  • S. Zühlke
  • M. Sapp
  • J. Nesme
  • S. J. Sørensen
  • Kornelia Smalla
  • Traud Winkelmann

Externe Organisationen

  • Technische Universität Braunschweig
  • Technische Universität Dortmund
  • Universitätsklinikum Düsseldorf
  • Københavns Universitet
  • Julius Kühn-Institut (JKI) Bundesforschungsinstitut für Kulturpflanzen
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer144
FachzeitschriftHorticulture Research
Jahrgang7
Ausgabenummer1
Frühes Online-Datum1 Sept. 2020
PublikationsstatusVeröffentlicht - 1 Dez. 2020

Abstract

Growth depression of Rosa plants at sites previously used to cultivate the same or closely related species is a typical symptom of rose replant disease (RRD). Currently, limited information is available on the causes and the etiology of RRD compared to apple replant disease (ARD). Thus, this study aimed at analyzing growth characteristics, root morphology, and root metabolites, as well as microbial communities in the rhizosphere of the susceptible rootstock Rosacorymbifera ‘Laxa’ grown in RRD-affected soil from two sites (Heidgraben and Sangerhausen), either untreated or disinfected by γ-irradiation. In a greenhouse bioassay, plants developed significantly more biomass in the γ-irradiated than in the untreated soils of both sites. Several plant metabolites detected in R. corymbifera ‘Laxa’ roots were site- and treatment-dependent. Although aloesin was recorded in significantly higher concentrations in untreated than in γ-irradiated soils from Heidgraben, the concentrations of phenylalanine were significantly lower in roots from untreated soil of both sites. Rhizosphere microbial communities of 8-week-old plants were studied by sequencing of 16S rRNA, ITS, and cox gene fragments amplified from total community DNA. Supported by microscopic observations, sequences affiliated to the bacterial genus Streptomyces and the fungal genus Nectria were identified as potential causal agents of RRD in the soils investigated. The relative abundance of oomycetes belonging to the genus Pythiogeton showed a negative correlation to the growth of the plants. Overall, the RRD symptoms, the effects of soil treatments on the composition of the rhizosphere microbial community revealed striking similarities to findings related to ARD.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Biotechnologie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Biochemie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Genetik
  • Agrar- und Biowissenschaften (insg.)
  • Pflanzenkunde
  • Agrar- und Biowissenschaften (insg.)
  • Gartenbau

Zitieren

Rhizosphere microbial communities associated to rose replant disease: links to plant growth and root metabolites. / Yim, B.; Baumann, A.; Grunewaldt-Stöcker, Gisela et al.
in: Horticulture Research, Jahrgang 7, Nr. 1, 144, 01.12.2020.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Yim, B, Baumann, A, Grunewaldt-Stöcker, G, Liu, B, Beerhues, L, Zühlke, S, Sapp, M, Nesme, J, Sørensen, SJ, Smalla, K & Winkelmann, T 2020, 'Rhizosphere microbial communities associated to rose replant disease: links to plant growth and root metabolites', Horticulture Research, Jg. 7, Nr. 1, 144. https://doi.org/10.1038/s41438-020-00365-2
Yim, B., Baumann, A., Grunewaldt-Stöcker, G., Liu, B., Beerhues, L., Zühlke, S., Sapp, M., Nesme, J., Sørensen, S. J., Smalla, K., & Winkelmann, T. (2020). Rhizosphere microbial communities associated to rose replant disease: links to plant growth and root metabolites. Horticulture Research, 7(1), Artikel 144. https://doi.org/10.1038/s41438-020-00365-2
Yim B, Baumann A, Grunewaldt-Stöcker G, Liu B, Beerhues L, Zühlke S et al. Rhizosphere microbial communities associated to rose replant disease: links to plant growth and root metabolites. Horticulture Research. 2020 Dez 1;7(1):144. Epub 2020 Sep 1. doi: 10.1038/s41438-020-00365-2
Yim, B. ; Baumann, A. ; Grunewaldt-Stöcker, Gisela et al. / Rhizosphere microbial communities associated to rose replant disease : links to plant growth and root metabolites. in: Horticulture Research. 2020 ; Jahrgang 7, Nr. 1.
Download
@article{21e0760dba0c4ecd9e05c6d0f3672424,
title = "Rhizosphere microbial communities associated to rose replant disease: links to plant growth and root metabolites",
abstract = "Growth depression of Rosa plants at sites previously used to cultivate the same or closely related species is a typical symptom of rose replant disease (RRD). Currently, limited information is available on the causes and the etiology of RRD compared to apple replant disease (ARD). Thus, this study aimed at analyzing growth characteristics, root morphology, and root metabolites, as well as microbial communities in the rhizosphere of the susceptible rootstock Rosacorymbifera {\textquoteleft}Laxa{\textquoteright} grown in RRD-affected soil from two sites (Heidgraben and Sangerhausen), either untreated or disinfected by γ-irradiation. In a greenhouse bioassay, plants developed significantly more biomass in the γ-irradiated than in the untreated soils of both sites. Several plant metabolites detected in R. corymbifera {\textquoteleft}Laxa{\textquoteright} roots were site- and treatment-dependent. Although aloesin was recorded in significantly higher concentrations in untreated than in γ-irradiated soils from Heidgraben, the concentrations of phenylalanine were significantly lower in roots from untreated soil of both sites. Rhizosphere microbial communities of 8-week-old plants were studied by sequencing of 16S rRNA, ITS, and cox gene fragments amplified from total community DNA. Supported by microscopic observations, sequences affiliated to the bacterial genus Streptomyces and the fungal genus Nectria were identified as potential causal agents of RRD in the soils investigated. The relative abundance of oomycetes belonging to the genus Pythiogeton showed a negative correlation to the growth of the plants. Overall, the RRD symptoms, the effects of soil treatments on the composition of the rhizosphere microbial community revealed striking similarities to findings related to ARD.",
author = "B. Yim and A. Baumann and Gisela Grunewaldt-St{\"o}cker and B. Liu and L. Beerhues and S. Z{\"u}hlke and M. Sapp and J. Nesme and S{\o}rensen, {S. J.} and Kornelia Smalla and Traud Winkelmann",
note = "Funding Information: We are deeply grateful for financial support by the Mathias-Tantau-Stiftung, Uetersen, Germany. B.L. and L.B. are also thankful to the Deutsche Forschungsgemeinschaft (DFG) for funding. We thank Florian Losch and Selahaddin Sezgin for instrumental analytical service, Dr. Andreas Wrede for providing us with both the stratified rose seeds originally obtained from Harald Klei nurseries and the soil from Heidgraben, and Thomas Hawel for providing us with the soil from Sangerhausen. We also thank our technicians for their help in the experimental setup and evaluations. Open access funding provided by Projekt DEAL.",
year = "2020",
month = dec,
day = "1",
doi = "10.1038/s41438-020-00365-2",
language = "English",
volume = "7",
journal = "Horticulture Research",
issn = "2052-7276",
publisher = "Nature Publishing Group",
number = "1",

}

Download

TY - JOUR

T1 - Rhizosphere microbial communities associated to rose replant disease

T2 - links to plant growth and root metabolites

AU - Yim, B.

AU - Baumann, A.

AU - Grunewaldt-Stöcker, Gisela

AU - Liu, B.

AU - Beerhues, L.

AU - Zühlke, S.

AU - Sapp, M.

AU - Nesme, J.

AU - Sørensen, S. J.

AU - Smalla, Kornelia

AU - Winkelmann, Traud

N1 - Funding Information: We are deeply grateful for financial support by the Mathias-Tantau-Stiftung, Uetersen, Germany. B.L. and L.B. are also thankful to the Deutsche Forschungsgemeinschaft (DFG) for funding. We thank Florian Losch and Selahaddin Sezgin for instrumental analytical service, Dr. Andreas Wrede for providing us with both the stratified rose seeds originally obtained from Harald Klei nurseries and the soil from Heidgraben, and Thomas Hawel for providing us with the soil from Sangerhausen. We also thank our technicians for their help in the experimental setup and evaluations. Open access funding provided by Projekt DEAL.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Growth depression of Rosa plants at sites previously used to cultivate the same or closely related species is a typical symptom of rose replant disease (RRD). Currently, limited information is available on the causes and the etiology of RRD compared to apple replant disease (ARD). Thus, this study aimed at analyzing growth characteristics, root morphology, and root metabolites, as well as microbial communities in the rhizosphere of the susceptible rootstock Rosacorymbifera ‘Laxa’ grown in RRD-affected soil from two sites (Heidgraben and Sangerhausen), either untreated or disinfected by γ-irradiation. In a greenhouse bioassay, plants developed significantly more biomass in the γ-irradiated than in the untreated soils of both sites. Several plant metabolites detected in R. corymbifera ‘Laxa’ roots were site- and treatment-dependent. Although aloesin was recorded in significantly higher concentrations in untreated than in γ-irradiated soils from Heidgraben, the concentrations of phenylalanine were significantly lower in roots from untreated soil of both sites. Rhizosphere microbial communities of 8-week-old plants were studied by sequencing of 16S rRNA, ITS, and cox gene fragments amplified from total community DNA. Supported by microscopic observations, sequences affiliated to the bacterial genus Streptomyces and the fungal genus Nectria were identified as potential causal agents of RRD in the soils investigated. The relative abundance of oomycetes belonging to the genus Pythiogeton showed a negative correlation to the growth of the plants. Overall, the RRD symptoms, the effects of soil treatments on the composition of the rhizosphere microbial community revealed striking similarities to findings related to ARD.

AB - Growth depression of Rosa plants at sites previously used to cultivate the same or closely related species is a typical symptom of rose replant disease (RRD). Currently, limited information is available on the causes and the etiology of RRD compared to apple replant disease (ARD). Thus, this study aimed at analyzing growth characteristics, root morphology, and root metabolites, as well as microbial communities in the rhizosphere of the susceptible rootstock Rosacorymbifera ‘Laxa’ grown in RRD-affected soil from two sites (Heidgraben and Sangerhausen), either untreated or disinfected by γ-irradiation. In a greenhouse bioassay, plants developed significantly more biomass in the γ-irradiated than in the untreated soils of both sites. Several plant metabolites detected in R. corymbifera ‘Laxa’ roots were site- and treatment-dependent. Although aloesin was recorded in significantly higher concentrations in untreated than in γ-irradiated soils from Heidgraben, the concentrations of phenylalanine were significantly lower in roots from untreated soil of both sites. Rhizosphere microbial communities of 8-week-old plants were studied by sequencing of 16S rRNA, ITS, and cox gene fragments amplified from total community DNA. Supported by microscopic observations, sequences affiliated to the bacterial genus Streptomyces and the fungal genus Nectria were identified as potential causal agents of RRD in the soils investigated. The relative abundance of oomycetes belonging to the genus Pythiogeton showed a negative correlation to the growth of the plants. Overall, the RRD symptoms, the effects of soil treatments on the composition of the rhizosphere microbial community revealed striking similarities to findings related to ARD.

UR - http://www.scopus.com/inward/record.url?scp=85090018074&partnerID=8YFLogxK

U2 - 10.1038/s41438-020-00365-2

DO - 10.1038/s41438-020-00365-2

M3 - Article

AN - SCOPUS:85090018074

VL - 7

JO - Horticulture Research

JF - Horticulture Research

SN - 2052-7276

IS - 1

M1 - 144

ER -

Von denselben Autoren