Response of a methane-driven interaction network to stressor intensification

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Adrian Ho
  • Lucas W Mendes
  • Hyo Jung Lee
  • Thomas Kaupper
  • Yongliang Mo
  • Anja Poehlein
  • Paul L E Bodelier
  • Zhongjun Jia
  • Marcus A Horn

Organisationseinheiten

Externe Organisationen

  • Universidade de Sao Paulo
  • Kunsan National University
  • Chinese Research Academy of Environmental Sciences (CRAES)
  • Netherlands Institute of Ecology (NIOO-KNAW)
  • Georg-August-Universität Göttingen
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummerfiaa180
FachzeitschriftFEMS microbiology ecology
Jahrgang96
Ausgabenummer10
Frühes Online-Datum28 Aug. 2020
PublikationsstatusVeröffentlicht - Okt. 2020

Abstract

Microorganisms may reciprocally select for specific interacting partners, forming a network with interdependent relationships. The methanotrophic interaction network, comprising methanotrophs and non-methanotrophs, is thought to modulate methane oxidation and give rise to emergent properties beneficial for the methanotrophs. Therefore, microbial interaction may become relevant for community functioning under stress. However, empirical validation of the role and stressor-induced response of the interaction network remains scarce. Here, we determined the response of a complex methane-driven interaction network to a stepwise increase in NH4Cl-induced stress (0.5-4.75 g L-1, in 0.25-0.5 g L-1 increments) using enrichment of a naturally occurring complex community derived from a paddy soil in laboratory-scale incubations. Although ammonium and intermediates of ammonium oxidation are known to inhibit methane oxidation, methanotrophic activity was unexpectedly detected even in incubations with high ammonium levels, albeit rates were significantly reduced. Sequencing analysis of the 16S rRNA and pmoA genes consistently revealed divergent communities in the reference and stressed incubations. The 16S rRNA-based co-occurrence network analysis revealed that NH4Cl-induced stress intensification resulted in a less complex and modular network, likely driven by less stable interaction. Interestingly, the non-methanotrophs formed the key nodes, and appear to be relevant members of the community. Overall, stressor intensification unravels the interaction network, with adverse consequences for community functioning.

ASJC Scopus Sachgebiete

Zitieren

Response of a methane-driven interaction network to stressor intensification. / Ho, Adrian; Mendes, Lucas W; Lee, Hyo Jung et al.
in: FEMS microbiology ecology, Jahrgang 96, Nr. 10, fiaa180, 10.2020.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Ho, A, Mendes, LW, Lee, HJ, Kaupper, T, Mo, Y, Poehlein, A, Bodelier, PLE, Jia, Z & Horn, MA 2020, 'Response of a methane-driven interaction network to stressor intensification', FEMS microbiology ecology, Jg. 96, Nr. 10, fiaa180. https://doi.org/10.1093/femsec/fiaa180
Ho, A., Mendes, L. W., Lee, H. J., Kaupper, T., Mo, Y., Poehlein, A., Bodelier, P. L. E., Jia, Z., & Horn, M. A. (2020). Response of a methane-driven interaction network to stressor intensification. FEMS microbiology ecology, 96(10), Artikel fiaa180. https://doi.org/10.1093/femsec/fiaa180
Ho A, Mendes LW, Lee HJ, Kaupper T, Mo Y, Poehlein A et al. Response of a methane-driven interaction network to stressor intensification. FEMS microbiology ecology. 2020 Okt;96(10):fiaa180. Epub 2020 Aug 28. doi: 10.1093/femsec/fiaa180
Ho, Adrian ; Mendes, Lucas W ; Lee, Hyo Jung et al. / Response of a methane-driven interaction network to stressor intensification. in: FEMS microbiology ecology. 2020 ; Jahrgang 96, Nr. 10.
Download
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AU - Ho, Adrian

AU - Mendes, Lucas W

AU - Lee, Hyo Jung

AU - Kaupper, Thomas

AU - Mo, Yongliang

AU - Poehlein, Anja

AU - Bodelier, Paul L E

AU - Jia, Zhongjun

AU - Horn, Marcus A

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N2 - Microorganisms may reciprocally select for specific interacting partners, forming a network with interdependent relationships. The methanotrophic interaction network, comprising methanotrophs and non-methanotrophs, is thought to modulate methane oxidation and give rise to emergent properties beneficial for the methanotrophs. Therefore, microbial interaction may become relevant for community functioning under stress. However, empirical validation of the role and stressor-induced response of the interaction network remains scarce. Here, we determined the response of a complex methane-driven interaction network to a stepwise increase in NH4Cl-induced stress (0.5-4.75 g L-1, in 0.25-0.5 g L-1 increments) using enrichment of a naturally occurring complex community derived from a paddy soil in laboratory-scale incubations. Although ammonium and intermediates of ammonium oxidation are known to inhibit methane oxidation, methanotrophic activity was unexpectedly detected even in incubations with high ammonium levels, albeit rates were significantly reduced. Sequencing analysis of the 16S rRNA and pmoA genes consistently revealed divergent communities in the reference and stressed incubations. The 16S rRNA-based co-occurrence network analysis revealed that NH4Cl-induced stress intensification resulted in a less complex and modular network, likely driven by less stable interaction. Interestingly, the non-methanotrophs formed the key nodes, and appear to be relevant members of the community. Overall, stressor intensification unravels the interaction network, with adverse consequences for community functioning.

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