TY - JOUR

T1 - Regeneration of miniature potted rose (Rosa hybrida L.) via somatic embryogenesis

AU - Zakizadeh, Hedayat

AU - Debener, Thomas

AU - Sriskandarajah, Sridevy

AU - Frello, Stefan

AU - Serek, Margrethe

PY - 2008/6

Y1 - 2008/6

N2 - Flowering plants were regenerated from in vitro grown leaves of miniature rose (Rosa hybrida L. 'Linda') via somatic embryogenesis. Sufficient amounts of callus were produced on in vitro leaves of 8 cultivars ('Leonie', 'Linda', 'Sonja', 'Toledo', 'Tiffany', 'Mette', 'Etna' and 'Andromeda') incubated on MS media fortified with either 45.2 or 90.5 μM 2,4- dichlorophenoxyacetic acid (2,4-D) for 6 weeks. Except for 'Tiffany' and 'Andromeda', embryogenic callus was induced on the callus of all cultivars incubated on MS media supplemented with different concentrations of zeatin or thidiazuron (TDZ). The highest frequency of primary somatic embryogenesis (30 %) was achieved with 'Sonja' on medium containing 22.7 μM TDZ, but the embryos from 'Sonja' failed to develop into plants. Only the somatic embryos of 'Linda' developed into flowering plants thus further work was carried out with this cultivar. The frequency of primary somatic embryogenesis in 'Linda' was 3.3 % on medium containing either 45.6 μM zeatin or 45.4 μM TDZ. The highest proliferation rate (3.8-fold) and the highest frequency of embryo maturation (76.6 %) were obtained on 1/2 MS medium fortified with 7.57 μM abscisic acid. Germination and subsequent shoot development on mature somatic embryos occurred in a medium containing 1.42 μM 1-naphthaleneacetic acid (NAA), 17.76 μM 6-benzylaminopurine (BAP) and 1.44 μM gibberellic acid (GA3). Shoots were rooted on 1/2 MS medium supplemented with 5.7 μM 3-indole acetic acid (IAA) and 9.84 μM indole-3-butyric acid (IBA) and transferred to the greenhouse. The plants flowered after about 10 weeks and were morphologically indistinguishable from the original plant.

AB - Flowering plants were regenerated from in vitro grown leaves of miniature rose (Rosa hybrida L. 'Linda') via somatic embryogenesis. Sufficient amounts of callus were produced on in vitro leaves of 8 cultivars ('Leonie', 'Linda', 'Sonja', 'Toledo', 'Tiffany', 'Mette', 'Etna' and 'Andromeda') incubated on MS media fortified with either 45.2 or 90.5 μM 2,4- dichlorophenoxyacetic acid (2,4-D) for 6 weeks. Except for 'Tiffany' and 'Andromeda', embryogenic callus was induced on the callus of all cultivars incubated on MS media supplemented with different concentrations of zeatin or thidiazuron (TDZ). The highest frequency of primary somatic embryogenesis (30 %) was achieved with 'Sonja' on medium containing 22.7 μM TDZ, but the embryos from 'Sonja' failed to develop into plants. Only the somatic embryos of 'Linda' developed into flowering plants thus further work was carried out with this cultivar. The frequency of primary somatic embryogenesis in 'Linda' was 3.3 % on medium containing either 45.6 μM zeatin or 45.4 μM TDZ. The highest proliferation rate (3.8-fold) and the highest frequency of embryo maturation (76.6 %) were obtained on 1/2 MS medium fortified with 7.57 μM abscisic acid. Germination and subsequent shoot development on mature somatic embryos occurred in a medium containing 1.42 μM 1-naphthaleneacetic acid (NAA), 17.76 μM 6-benzylaminopurine (BAP) and 1.44 μM gibberellic acid (GA3). Shoots were rooted on 1/2 MS medium supplemented with 5.7 μM 3-indole acetic acid (IAA) and 9.84 μM indole-3-butyric acid (IBA) and transferred to the greenhouse. The plants flowered after about 10 weeks and were morphologically indistinguishable from the original plant.

KW - Callus

KW - Plant growth regulator

KW - Plant regeneration

KW - Rose

KW - Somatic embryos

UR - http://www.scopus.com/inward/record.url?scp=46849107135&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:46849107135

VL - 73

SP - 111

EP - 117

JO - European Journal of Horticultural Science

JF - European Journal of Horticultural Science

SN - 1611-4434

IS - 3

ER -