TY - JOUR

T1 - Regeneration from phylloclade explants and callus cultures of Schlumbergera and Rhipsalidopsis

AU - Sriskandarajah, Sridevy

AU - Serek, Margrethe

N1 - Funding Information: The project was funded by the Danish Ministry of Agriculture and Fisheries (93s-2466-Å01-01430) and Danish Schlumbergera Growers and Breeders: Gartneriet Thoruplund A/S (Odense), Rohdes Gartneri A/S (Kerteminde), Gartneriet PKM ApS (Odense), and Hansson DK (Søndersø). The authors would like to thank Drs Traud Winkelmann and Stefan Frello for critical review of the manuscript.

PY - 2004/7

Y1 - 2004/7

N2 - Phylloclade explants of Schlumbergera and Rhipsalidopsis were cultured in vitro to produce axillary and adventitious shoots. The explants of both species, taken from greenhouse-grown plants, produced only axillary shoots. There was a pronounced improvement in adventitious shoot formation in phylloclade explants of cultivar CB4 of Rhipsalidopsis by increasing numbers of subcultures of axillary shoots used as donor plants. The axillary shoots generated from the explants were either subcultured to produce successive generations of axillary shoot cultures or made into phylloclade explants and tested for adventitious shoot formation at each subculture. The duration of each subculture varied from 6 to 12 weeks. After the first subculture, sporadic adventitious shoot formation began, and after the third subculture 87% explants of cultivar CB4 produced adventitious shoots at a frequency of about 12 shoots per explant. In contrast, there was no improvement in regenerative ability in explants of cultivar Thor-Olga of Schlumbergera up to third subculture. Adventitious shoots could be produced by callus culture too. Cultivar CB4 was highly regenerative, producing as many as 10 adventitious shoots per square cm of callus. In vitro grown plantlets, when transferred to pots continued to show prolific growth.

AB - Phylloclade explants of Schlumbergera and Rhipsalidopsis were cultured in vitro to produce axillary and adventitious shoots. The explants of both species, taken from greenhouse-grown plants, produced only axillary shoots. There was a pronounced improvement in adventitious shoot formation in phylloclade explants of cultivar CB4 of Rhipsalidopsis by increasing numbers of subcultures of axillary shoots used as donor plants. The axillary shoots generated from the explants were either subcultured to produce successive generations of axillary shoot cultures or made into phylloclade explants and tested for adventitious shoot formation at each subculture. The duration of each subculture varied from 6 to 12 weeks. After the first subculture, sporadic adventitious shoot formation began, and after the third subculture 87% explants of cultivar CB4 produced adventitious shoots at a frequency of about 12 shoots per explant. In contrast, there was no improvement in regenerative ability in explants of cultivar Thor-Olga of Schlumbergera up to third subculture. Adventitious shoots could be produced by callus culture too. Cultivar CB4 was highly regenerative, producing as many as 10 adventitious shoots per square cm of callus. In vitro grown plantlets, when transferred to pots continued to show prolific growth.

KW - Adventitious shoots

KW - Christmas cactus

KW - Easter cactus

KW - in vitro culture

KW - Subculture

UR - http://www.scopus.com/inward/record.url?scp=1842686707&partnerID=8YFLogxK

U2 - 10.1023/B:TICU.0000020398.11717.88

DO - 10.1023/B:TICU.0000020398.11717.88

M3 - Article

AN - SCOPUS:1842686707

VL - 78

SP - 75

EP - 81

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -