Recovery of extracellular alkaline lipases of Fusarium spec. by foam fractionation

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Janaina N. Dos Prazeres
  • Ana P. Simiqueli
  • Glaucia M. Pastore
  • Diana Linke
  • Holger Zorn
  • Manfred Nimtz
  • Ralf G. Berger

Organisationseinheiten

Externe Organisationen

  • Universidade Estadual de Campinas
  • Technische Universität Dortmund
  • Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
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Details

OriginalspracheEnglisch
Seiten (von - bis)1503-1508
Seitenumfang6
FachzeitschriftFresenius environmental bulletin
Jahrgang16
Ausgabenummer11 B
PublikationsstatusVeröffentlicht - 2007

Abstract

Foam fractionation was used to concentrate and to purify extracellular alkaline lipases produced by Fusarium spec. in submerged cultures supplemented with Tween 80. Gas flow rate and pH-value proved to be crucial process parameters. Maximum recovery rates of lipolytic activity were obtained at 60 mL min-1 N2 and pH 8.5. Under these conditions, > 90% of the total lipase activity was recovered in the foam with enrichment and purification factors of 8.8 and 5.9, respectively. The secreted lipases were characterized biochemically by means of IEF electrophoresis with activity staining and electrospray tandem mass spectrometry.

ASJC Scopus Sachgebiete

Zitieren

Recovery of extracellular alkaline lipases of Fusarium spec. by foam fractionation. / Dos Prazeres, Janaina N.; Simiqueli, Ana P.; Pastore, Glaucia M. et al.
in: Fresenius environmental bulletin, Jahrgang 16, Nr. 11 B, 2007, S. 1503-1508.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Dos Prazeres, JN, Simiqueli, AP, Pastore, GM, Linke, D, Zorn, H, Nimtz, M & Berger, RG 2007, 'Recovery of extracellular alkaline lipases of Fusarium spec. by foam fractionation', Fresenius environmental bulletin, Jg. 16, Nr. 11 B, S. 1503-1508.
Dos Prazeres, J. N., Simiqueli, A. P., Pastore, G. M., Linke, D., Zorn, H., Nimtz, M., & Berger, R. G. (2007). Recovery of extracellular alkaline lipases of Fusarium spec. by foam fractionation. Fresenius environmental bulletin, 16(11 B), 1503-1508.
Dos Prazeres JN, Simiqueli AP, Pastore GM, Linke D, Zorn H, Nimtz M et al. Recovery of extracellular alkaline lipases of Fusarium spec. by foam fractionation. Fresenius environmental bulletin. 2007;16(11 B):1503-1508.
Dos Prazeres, Janaina N. ; Simiqueli, Ana P. ; Pastore, Glaucia M. et al. / Recovery of extracellular alkaline lipases of Fusarium spec. by foam fractionation. in: Fresenius environmental bulletin. 2007 ; Jahrgang 16, Nr. 11 B. S. 1503-1508.
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Download

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T1 - Recovery of extracellular alkaline lipases of Fusarium spec. by foam fractionation

AU - Dos Prazeres, Janaina N.

AU - Simiqueli, Ana P.

AU - Pastore, Glaucia M.

AU - Linke, Diana

AU - Zorn, Holger

AU - Nimtz, Manfred

AU - Berger, Ralf G.

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KW - Alkaline lipase

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KW - Fusarium spec.

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JO - Fresenius environmental bulletin

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