TY - JOUR

T1 - Recovery aptitude of the halophyte Cakile maritima upon water deficit stress release is sustained by extensive modulation of the leaf proteome

AU - Farhat, Nèjia

AU - Belghith, Ikram

AU - Senkler, Jennifer

AU - Hichri, Sarra

AU - Abdelly, Chedly

AU - Braun, Hans-Peter

AU - Debez, Ahmed

N1 - Funding Information: This work was supported by the German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD)in the framework of the project “Proteomics and Halophyte Stress Tolerance” (ID 57247769)of the “Transformation partnership-Line 4″ program. We thank Tatiana Hildebrandt, Marianne Langer, and Patrick Künzler for their active assistance regarding gel image analysis, protein digestion, and immunoblotting, respectively.

PY - 2019/9/15

Y1 - 2019/9/15

N2 - Among the most intriguing features characterizing extremophile plants is their ability to rapidly recover growth activity upon stress release. Here, we investigated the responses of the halophyte C. maritima to drought and recovery at both physiological and leaf proteome levels. Six week-old plants were either cultivated at 100% or at 25% field capacity. After 12 d of treatment, one lot of dehydrated plants was rewatered to 100% FC for 14 d (stress release). Drought stress impaired shoot hydration, photosynthetic activity and chlorophyll content compared to the control, resulting in severe plant growth restriction. This was concomitant with a marked increase in anthocyanin and proline concentrations. Upon stress release, C. maritima rapidly recovered with respect to all measured parameters. Two-dimensional gel-based proteome analysis of leaves revealed 84 protein spots with significantly changed volumes at the compared conditions: twenty-eight protein spots between normally watered plants and stressed plants but even 70 proteins between stressed and recovered plants. Proteins with higher abundance induced upon rewatering were mostly involved in photosynthesis, glycolytic pathway, TCA cycle, protein biosynthesis, and other metabolic pathways. Overall, C. maritima likely adopts a drought-avoidance strategy, involving efficient mechanisms specifically taking place upon stress release, leading to fast and strong recovery.

AB - Among the most intriguing features characterizing extremophile plants is their ability to rapidly recover growth activity upon stress release. Here, we investigated the responses of the halophyte C. maritima to drought and recovery at both physiological and leaf proteome levels. Six week-old plants were either cultivated at 100% or at 25% field capacity. After 12 d of treatment, one lot of dehydrated plants was rewatered to 100% FC for 14 d (stress release). Drought stress impaired shoot hydration, photosynthetic activity and chlorophyll content compared to the control, resulting in severe plant growth restriction. This was concomitant with a marked increase in anthocyanin and proline concentrations. Upon stress release, C. maritima rapidly recovered with respect to all measured parameters. Two-dimensional gel-based proteome analysis of leaves revealed 84 protein spots with significantly changed volumes at the compared conditions: twenty-eight protein spots between normally watered plants and stressed plants but even 70 proteins between stressed and recovered plants. Proteins with higher abundance induced upon rewatering were mostly involved in photosynthesis, glycolytic pathway, TCA cycle, protein biosynthesis, and other metabolic pathways. Overall, C. maritima likely adopts a drought-avoidance strategy, involving efficient mechanisms specifically taking place upon stress release, leading to fast and strong recovery.

KW - Cakile maritima

KW - Drought

KW - Halophyte

KW - Leaves

KW - Proteomics

KW - Stress release

UR - http://www.scopus.com/inward/record.url?scp=85064860822&partnerID=8YFLogxK

U2 - 10.1016/j.ecoenv.2019.04.072

DO - 10.1016/j.ecoenv.2019.04.072

M3 - Article

C2 - 31048216

AN - SCOPUS:85064860822

VL - 179

SP - 198

EP - 211

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

ER -