TY - JOUR

T1 - Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

AU - Hobisch, Markus

AU - Holtmann, Dirk

AU - Gomez de Santos, Patricia

AU - Alcalde, Miguel

AU - Hollmann, Frank

AU - Kara, Selin

N1 - Funding Information: This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk?odowska-Curie grant agreement No 764920. MA and PGS thank the FPI fellowship BES-2017-080040, the Spanish Government Project PID2019-106166RB-I00-Oxywave, and the Comunidad de Madrid Synergy CAM project Y2018/BIO-4738-EVOCHIMERA-CM. FH gratefully acknowledges support by The Netherlands Organisation for Scientific Research through a VICI grant (no. 724.014.003) and the European Research Commission (ERC consolidator grant No 648026).

PY - 2021/11/1

Y1 - 2021/11/1

N2 - Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.

AB - Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.

KW - Fungal enzyme

KW - Oxidation chemistry

KW - Oxyfunctionalisation

KW - Oxygenase

KW - Peroxygenase

UR - http://www.scopus.com/inward/record.url?scp=85089826545&partnerID=8YFLogxK

U2 - 10.1016/j.biotechadv.2020.107615

DO - 10.1016/j.biotechadv.2020.107615

M3 - Review article

C2 - 32827669

AN - SCOPUS:85089826545

VL - 51

JO - Biotechnology advances

JF - Biotechnology advances

SN - 0734-9750

M1 - 107615

ER -