TY - JOUR

T1 - RecA-dependent viral burst in bacterial colonies during the entry into stationary phase

AU - Ramírez, E.

AU - Schmidt, M.

AU - Rinas, U.

AU - Villaverde, A.

N1 - Funding Information: We are indebted to A. Poteete for generously providing wild-type P22, to the Salmonella Genetic Stock Center for strain SA2386, and to J. Checa and V. Ferreres for technical assistance. This work has been supported by Grants BIO95-0801 and BIO98-0527 (CICYT), HA96-0028 and HA97-0136 (MEC), by the DAAD (Acciones Integradas 314-A1-e-dr) and by the M a Francisca de Roviralta Foundation. E.R. is the recipient of a predoctoral fellowship from MEC, Spain.

PY - 1999/1/15

Y1 - 1999/1/15

N2 - We have explored the nature of the sudden viral amplification observed during the ageing of P22-infected lysogenic colonies of Salmonella typhimurium [Ramirez, E, and Villaverde, A. (1997) Gene 202, 147-149]. By a comparative analysis of the wild-type P22 and a P22 integration mutant, it has been shown that the conditions promoting prophage induction occur in only a small portion of the bacterial population and briefly during the transition between the exponential growth and the stationary phase. The viral burst is RecA-dependent and cannot be reproduced in continuous culture by a mere decrease of the growth rate. This suggests that the limited viral propagation in colonies is probably linked to heterogeneous physiological conditions within colonial populations, distinct from those of the homogeneous liquid cultures. Copyright (C) 1999 Federation of European Microbiological Societies.

AB - We have explored the nature of the sudden viral amplification observed during the ageing of P22-infected lysogenic colonies of Salmonella typhimurium [Ramirez, E, and Villaverde, A. (1997) Gene 202, 147-149]. By a comparative analysis of the wild-type P22 and a P22 integration mutant, it has been shown that the conditions promoting prophage induction occur in only a small portion of the bacterial population and briefly during the transition between the exponential growth and the stationary phase. The viral burst is RecA-dependent and cannot be reproduced in continuous culture by a mere decrease of the growth rate. This suggests that the limited viral propagation in colonies is probably linked to heterogeneous physiological conditions within colonial populations, distinct from those of the homogeneous liquid cultures. Copyright (C) 1999 Federation of European Microbiological Societies.

KW - Colony growth

KW - Growth rate

KW - P22

KW - Prophage induction

KW - RecA

KW - Salmonella

UR - http://www.scopus.com/inward/record.url?scp=0344766110&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(98)00559-X

DO - 10.1016/S0378-1097(98)00559-X

M3 - Article

C2 - 9933926

AN - SCOPUS:0344766110

VL - 170

SP - 313

EP - 317

JO - FEMS microbiology letters

JF - FEMS microbiology letters

SN - 0378-1097

IS - 2

ER -