Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 16635-16641 |
| Seitenumfang | 7 |
| Fachzeitschrift | Journal of the American Chemical Society |
| Jahrgang | 133 |
| Ausgabenummer | 41 |
| Publikationsstatus | Veröffentlicht - 19 Okt. 2011 |
| Extern publiziert | Ja |
Abstract
The mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. We conducted rational domain swaps between the polyketide synthases encoding the biosynthesis of the closely related compounds tenellin and desmethylbassianin. Expression of the hybrid synthetases in Aspergillus oryzae led to the production of reprogrammed compounds in which the changes to the methylation pattern and chain length could be mapped to the domain swaps. These experiments reveal for the first time the origin of programming in these systems. Domain swaps combined with coexpression of two cytochrome P450 encoding genes from the tenellin biosynthetic gene cluster led to the resurrection of the extinct metabolite bassianin.
ASJC Scopus Sachgebiete
- Chemische Verfahrenstechnik (insg.)
- Katalyse
- Chemie (insg.)
- Allgemeine Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Chemische Verfahrenstechnik (insg.)
- Kolloid- und Oberflächenchemie
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in: Journal of the American Chemical Society, Jahrgang 133, Nr. 41, 19.10.2011, S. 16635-16641.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Rational domain swaps decipher programming in fungal highly reducing polyketide synthases and resurrect an extinct metabolite
AU - Fisch, Katja M.
AU - Bakeer, Walid
AU - Yakasai, Ahmed A.
AU - Song, Zhongshu
AU - Pedrick, Jennifer
AU - Wasil, Zahida
AU - Bailey, Andrew M.
AU - Lazarus, Colin M.
AU - Simpson, Thomas J.
AU - Cox, Russell J.
PY - 2011/10/19
Y1 - 2011/10/19
N2 - The mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. We conducted rational domain swaps between the polyketide synthases encoding the biosynthesis of the closely related compounds tenellin and desmethylbassianin. Expression of the hybrid synthetases in Aspergillus oryzae led to the production of reprogrammed compounds in which the changes to the methylation pattern and chain length could be mapped to the domain swaps. These experiments reveal for the first time the origin of programming in these systems. Domain swaps combined with coexpression of two cytochrome P450 encoding genes from the tenellin biosynthetic gene cluster led to the resurrection of the extinct metabolite bassianin.
AB - The mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. We conducted rational domain swaps between the polyketide synthases encoding the biosynthesis of the closely related compounds tenellin and desmethylbassianin. Expression of the hybrid synthetases in Aspergillus oryzae led to the production of reprogrammed compounds in which the changes to the methylation pattern and chain length could be mapped to the domain swaps. These experiments reveal for the first time the origin of programming in these systems. Domain swaps combined with coexpression of two cytochrome P450 encoding genes from the tenellin biosynthetic gene cluster led to the resurrection of the extinct metabolite bassianin.
UR - http://www.scopus.com/inward/record.url?scp=80054760185&partnerID=8YFLogxK
U2 - 10.1021/ja206914q
DO - 10.1021/ja206914q
M3 - Article
C2 - 21899331
AN - SCOPUS:80054760185
VL - 133
SP - 16635
EP - 16641
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 41
ER -