TY - JOUR

T1 - Rapid Microfluidic Preparation of Niosomes for Targeted Drug Delivery

AU - Seleci, Didem Ag

AU - Maurer, Viktor

AU - Stahl, Frank

AU - Scheper, Thomas

AU - Garnweitner, Georg

N1 - Funding information: This research received no external funding. Publication of the results was supported by the German Research Foundation and the Open Access Publication Funds of the Technische Universität Braunschweig. This research received no external funding. Publication of the results was supported by the German Research Foundation and the Open Access Publication Funds of the Technische Universit?t Braunschweig. The authors thank Bilal Temel, Technische Universit?t Braunschweig, and the Laboratory of Nano and Quantum Engineering (LNQE) of the Leibniz University of Hannover for the TEM measurements. We acknowledge support by the German Research Foundation and the Open Access Publication Funds of the Technische Universit?t Braunschweig.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Niosomes are non-ionic surfactant-based vesicles with high promise for drug delivery applications. They can be rapidly prepared via microfluidics, allowing their reproducible production without the need of a subsequent size reduction step, by controlled mixing of two miscible phases of an organic (lipids dissolved in alcohol) and an aqueous solution in a microchannel. The control of niosome properties and the implementation of more complex functions, however, thus far are largely unknown for this method. Here we investigate microfluidics-based manufacturing of topotecan (TPT)-loaded polyethylene glycolated niosomes (PEGNIO). The flow rate ratio of the organic and aqueous phases was varied and optimized. Furthermore, the surface of TPT-loaded PEGNIO was modified with a tumor homing and penetrating peptide (tLyp-1). The designed nanoparticular drug delivery system composed of PEGNIO-TPT-tLyp-1 was fabricated for the first time via microfluidics in this study. The physicochemical properties were determined through dynamic light scattering (DLS) and zeta potential analysis. In vitro studies of the obtained formulations were performed on human glioblastoma (U87) cells. The results clearly indicated that tLyp-1-functionalized TPT-loaded niosomes could significantly improve anti-glioma treatment.

AB - Niosomes are non-ionic surfactant-based vesicles with high promise for drug delivery applications. They can be rapidly prepared via microfluidics, allowing their reproducible production without the need of a subsequent size reduction step, by controlled mixing of two miscible phases of an organic (lipids dissolved in alcohol) and an aqueous solution in a microchannel. The control of niosome properties and the implementation of more complex functions, however, thus far are largely unknown for this method. Here we investigate microfluidics-based manufacturing of topotecan (TPT)-loaded polyethylene glycolated niosomes (PEGNIO). The flow rate ratio of the organic and aqueous phases was varied and optimized. Furthermore, the surface of TPT-loaded PEGNIO was modified with a tumor homing and penetrating peptide (tLyp-1). The designed nanoparticular drug delivery system composed of PEGNIO-TPT-tLyp-1 was fabricated for the first time via microfluidics in this study. The physicochemical properties were determined through dynamic light scattering (DLS) and zeta potential analysis. In vitro studies of the obtained formulations were performed on human glioblastoma (U87) cells. The results clearly indicated that tLyp-1-functionalized TPT-loaded niosomes could significantly improve anti-glioma treatment.

KW - Glioma

KW - Microfluidics

KW - Niosomes

KW - Targeted drug delivery

UR - http://www.scopus.com/inward/record.url?scp=85073504950&partnerID=8YFLogxK

U2 - 10.3390/ijms20194696

DO - 10.3390/ijms20194696

M3 - Article

C2 - 31546717

AN - SCOPUS:85073504950

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 19

M1 - 4696

ER -