TY - GEN

T1 - Rapid antifungal susceptibility testing on silicon microwells

AU - Heuer, Christopher

AU - Leonard, Heidi

AU - Nitzan, Nadav

AU - Lavy-Alperovitch, Ariella

AU - Massad-Ivanir, Naama

AU - Bahnemann, Janina

AU - Scheper, Thomas

AU - Segal, Ester

N1 - Funding information: This work was partially supported by the Israel Innovation Authority. CH acknowledges the financial support from the Leibniz Universitätsgesellschaft and the Technion. This work was partially supported by the Israel Innovation Authority. CH acknowledges the financial support from the Leibniz Universit?tsgesellschaft and the Technion.

PY - 2020

Y1 - 2020

N2 - In this work, we present a sensing system for rapid antifungal susceptibility testing (AFST). The sensor is based on silicon-based diffraction gratings; their microstructure allows to capture the fungal conidia and monitor their subsequent growth on a solid-liquid interface. As a proof of concept, we use Aspergillus Niger (A. Niger) and study its growth and response to common antifungals (at varying concentrations) by monitoring changes in the intensity of reflected light from the grating. The assay is employed for the determination of minimum inhibitory concentration (MIC) values and allows expeditious AFST within 12 h, compared to ~1-2 days using gold standard methods.

AB - In this work, we present a sensing system for rapid antifungal susceptibility testing (AFST). The sensor is based on silicon-based diffraction gratings; their microstructure allows to capture the fungal conidia and monitor their subsequent growth on a solid-liquid interface. As a proof of concept, we use Aspergillus Niger (A. Niger) and study its growth and response to common antifungals (at varying concentrations) by monitoring changes in the intensity of reflected light from the grating. The assay is employed for the determination of minimum inhibitory concentration (MIC) values and allows expeditious AFST within 12 h, compared to ~1-2 days using gold standard methods.

KW - Antifungal Susceptibility Testing

KW - Sensors

KW - Silicon Microwell Diffraction Gratings

UR - http://www.scopus.com/inward/record.url?scp=85098262497&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85098262497

T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 601

EP - 602

BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020

Y2 - 4 October 2020 through 9 October 2020

ER -