TY - JOUR

T1 - Protoplast isolation and plant regeneration of different genotypes of Petunia and calibrachoa

AU - Meyer, L.

AU - Serek, M.

AU - Winkelmann, T.

N1 - Funding Information: Acknowledgments This project was funded by the Federal Ministry of Economy and Technology (BMWi). The authors thank Dr. Andrea Dohm, breeding director Selecta Klemm GmbH, for excellent cooperation during this study, and Professor Errol Hewett (Massey University, Palmerston North, New Zealand) for linguistic editing of the manuscript.

PY - 2009/8/8

Y1 - 2009/8/8

N2 - Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.

AB - Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.

KW - Callus formation

KW - Plant regeneration and stability

KW - Protoplast yield

UR - http://www.scopus.com/inward/record.url?scp=70350196392&partnerID=8YFLogxK

U2 - 10.1007/s11240-009-9572-4

DO - 10.1007/s11240-009-9572-4

M3 - Article

AN - SCOPUS:70350196392

VL - 99

SP - 27

EP - 34

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -