Protocol for the application of single-cell damage in murine intestinal organoid models

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Anna Elisabeth Seidler
  • Sören Donath
  • Lara Gentemann
  • Manuela Buettner
  • Alexander Heisterkamp
  • Stefan Kalies

Organisationseinheiten

Externe Organisationen

  • NIFE- Niedersächsisches Zentrum für Biomedizintechnik, Implantatforschung und Entwicklung
  • REBIRTH Forschungszentrum für translationale regenerative Medizin
  • Medizinische Hochschule Hannover (MHH)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer103153
Seitenumfang31
FachzeitschriftSTAR Protocols
Jahrgang5
Ausgabenummer3
Frühes Online-Datum30 Juli 2024
PublikationsstatusVeröffentlicht - 20 Sept. 2024

Abstract

Spatially defined organoid damage enables the study of cellular repair processes. However, capturing dynamic events in living tissues is technically challenging. Here, we present a protocol for the application of single-cell damage in intestinal organoid models. We describe steps for isolating and cultivating murine colon organoids, lentivirus generation and transduction of organoids, single-cell ablation by a femtosecond laser, and follow-up imaging analysis. We provide examples for the image acquisition pipeline of dynamic processes in organoids using a confocal microscope. For complete details on the use and execution of this protocol, please refer to Donath et al.1,2

ASJC Scopus Sachgebiete

Zitieren

Protocol for the application of single-cell damage in murine intestinal organoid models. / Seidler, Anna Elisabeth; Donath, Sören; Gentemann, Lara et al.
in: STAR Protocols, Jahrgang 5, Nr. 3, 103153, 20.09.2024.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Seidler, AE, Donath, S, Gentemann, L, Buettner, M, Heisterkamp, A & Kalies, S 2024, 'Protocol for the application of single-cell damage in murine intestinal organoid models', STAR Protocols, Jg. 5, Nr. 3, 103153. https://doi.org/10.1016/j.xpro.2024.103153
Seidler, A. E., Donath, S., Gentemann, L., Buettner, M., Heisterkamp, A., & Kalies, S. (2024). Protocol for the application of single-cell damage in murine intestinal organoid models. STAR Protocols, 5(3), Artikel 103153. https://doi.org/10.1016/j.xpro.2024.103153
Seidler AE, Donath S, Gentemann L, Buettner M, Heisterkamp A, Kalies S. Protocol for the application of single-cell damage in murine intestinal organoid models. STAR Protocols. 2024 Sep 20;5(3):103153. Epub 2024 Jul 30. doi: 10.1016/j.xpro.2024.103153
Seidler, Anna Elisabeth ; Donath, Sören ; Gentemann, Lara et al. / Protocol for the application of single-cell damage in murine intestinal organoid models. in: STAR Protocols. 2024 ; Jahrgang 5, Nr. 3.
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