Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 787-794 |
| Seitenumfang | 8 |
| Fachzeitschrift | Journal of Plant Physiology |
| Jahrgang | 158 |
| Ausgabenummer | 6 |
| Publikationsstatus | Veröffentlicht - 1 Jan. 2001 |
Abstract
The alloplasmic 'Tournefortii' CMS system from B. napus was chosen for a comprehensive investigation of the protein content of the mitochondrial compartment in male sterile and fertile near isogenic lines. As proteins involved in the male sterile phenotype may be expressed in a tissue-specific manner, mitochondrial preparations were made from different tissues and checked for the purity of the organelles with a new and highly sensitive method (BN-PAGE). In Brassica, only mitochondrial preparations from 4-5-day-old seedlings and from flower buds were found to be sufficiently pure. High resolution, two-dimensional gel electrophoresis of total protein from anthers and from mitochondrial protein of male sterile and fertile seedlings of near isogenic lines revealed distinct differences in the protein patterns. Several explanations for the occurrence of these differences, which may be the starting point for a detailed molecular analysis and a better understanding of the CMS syndrome, are discussed.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Physiologie
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Journal of Plant Physiology, Jahrgang 158, Nr. 6, 01.01.2001, S. 787-794.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Proteomic approach for investigation of cytoplasmic male sterility (CMS) in Brassica
AU - Mihr, Christina
AU - Baumgärtner, Maja
AU - Dieterich, Jens Holger
AU - Schmitz, Udo
AU - Braun, Hans-Peter
N1 - Funding information:. We wish to thank Drs. H. Becker, G. Stiewe, W. Ecke, and C. Möllers, Göttingen, for providing plant material of B. napus. Thanks are also due to D. Lewejohann and G. Kühne for expert technical assistance. All the experiments described in this paper comply with the current laws of Germany. This work was supported by a grant to UKS, from the Deutsche Forschungsgemeinschaft (Schm 698/ 8-1), and a grant to HPB from the Fonds der Chemischen Industrie.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - The alloplasmic 'Tournefortii' CMS system from B. napus was chosen for a comprehensive investigation of the protein content of the mitochondrial compartment in male sterile and fertile near isogenic lines. As proteins involved in the male sterile phenotype may be expressed in a tissue-specific manner, mitochondrial preparations were made from different tissues and checked for the purity of the organelles with a new and highly sensitive method (BN-PAGE). In Brassica, only mitochondrial preparations from 4-5-day-old seedlings and from flower buds were found to be sufficiently pure. High resolution, two-dimensional gel electrophoresis of total protein from anthers and from mitochondrial protein of male sterile and fertile seedlings of near isogenic lines revealed distinct differences in the protein patterns. Several explanations for the occurrence of these differences, which may be the starting point for a detailed molecular analysis and a better understanding of the CMS syndrome, are discussed.
AB - The alloplasmic 'Tournefortii' CMS system from B. napus was chosen for a comprehensive investigation of the protein content of the mitochondrial compartment in male sterile and fertile near isogenic lines. As proteins involved in the male sterile phenotype may be expressed in a tissue-specific manner, mitochondrial preparations were made from different tissues and checked for the purity of the organelles with a new and highly sensitive method (BN-PAGE). In Brassica, only mitochondrial preparations from 4-5-day-old seedlings and from flower buds were found to be sufficiently pure. High resolution, two-dimensional gel electrophoresis of total protein from anthers and from mitochondrial protein of male sterile and fertile seedlings of near isogenic lines revealed distinct differences in the protein patterns. Several explanations for the occurrence of these differences, which may be the starting point for a detailed molecular analysis and a better understanding of the CMS syndrome, are discussed.
KW - 2-D-gel electrophoresis
KW - Cytoplasmic male sterility
KW - Proteomics mitochondria
KW - Rape
KW - Seed (Brassica napus L.)
UR - http://www.scopus.com/inward/record.url?scp=0034957642&partnerID=8YFLogxK
U2 - 10.1078/0176-1617-00292
DO - 10.1078/0176-1617-00292
M3 - Article
AN - SCOPUS:0034957642
VL - 158
SP - 787
EP - 794
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
IS - 6
ER -