Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • Patrick Römer
  • Sabine Recht
  • Tina Strauß
  • Janett Elsaesser
  • Sebastian Schornack
  • Jens Boch
  • Shiping Wang
  • Thomas Lahaye

Externe Organisationen

  • Martin-Luther-Universität Halle-Wittenberg
  • Ludwig-Maximilians-Universität München (LMU)
  • The Sainsbury Laboratory (TSL)
  • Huazhong Agricultural University
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)1048-1057
Seitenumfang10
FachzeitschriftNew Phytologist
Jahrgang187
Ausgabenummer4
PublikationsstatusVeröffentlicht - 12 Aug. 2010
Extern publiziertJa

Abstract

•Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes.•Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via β-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA).•We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes.•Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.

ASJC Scopus Sachgebiete

Zitieren

Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae. / Römer, Patrick; Recht, Sabine; Strauß, Tina et al.
in: New Phytologist, Jahrgang 187, Nr. 4, 12.08.2010, S. 1048-1057.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Römer P, Recht S, Strauß T, Elsaesser J, Schornack S, Boch J et al. Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae. New Phytologist. 2010 Aug 12;187(4):1048-1057. doi: 10.1111/j.1469-8137.2010.03217.x
Download
@article{698d81e6e6f042deb79cbafda62324a1,
title = "Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae",
abstract = "•Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes.•Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via β-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA).•We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes.•Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.",
keywords = "AvrBs3, AvrXa27, Bs3, PthXo1, UPA (up-regulated by AvrBs3), Xa13, Xanthomonas",
author = "Patrick R{\"o}mer and Sabine Recht and Tina Strau{\ss} and Janett Elsaesser and Sebastian Schornack and Jens Boch and Shiping Wang and Thomas Lahaye",
note = "Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",
year = "2010",
month = aug,
day = "12",
doi = "10.1111/j.1469-8137.2010.03217.x",
language = "English",
volume = "187",
pages = "1048--1057",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "4",

}

Download

TY - JOUR

T1 - Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae

AU - Römer, Patrick

AU - Recht, Sabine

AU - Strauß, Tina

AU - Elsaesser, Janett

AU - Schornack, Sebastian

AU - Boch, Jens

AU - Wang, Shiping

AU - Lahaye, Thomas

N1 - Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2010/8/12

Y1 - 2010/8/12

N2 - •Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes.•Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via β-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA).•We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes.•Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.

AB - •Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes.•Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via β-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA).•We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes.•Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.

KW - AvrBs3

KW - AvrXa27

KW - Bs3

KW - PthXo1

KW - UPA (up-regulated by AvrBs3)

KW - Xa13

KW - Xanthomonas

UR - http://www.scopus.com/inward/record.url?scp=77955737033&partnerID=8YFLogxK

U2 - 10.1111/j.1469-8137.2010.03217.x

DO - 10.1111/j.1469-8137.2010.03217.x

M3 - Article

C2 - 20345643

AN - SCOPUS:77955737033

VL - 187

SP - 1048

EP - 1057

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 4

ER -