Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1126-1136 |
| Seitenumfang | 11 |
| Fachzeitschrift | Journal of Biomaterials Applications |
| Jahrgang | 36 |
| Ausgabenummer | 6 |
| Frühes Online-Datum | 7 Okt. 2021 |
| Publikationsstatus | Veröffentlicht - Jan. 2022 |
Abstract
Objective: The generation of bio-/hemocompatible cardiovascular patches with sufficient stability and regenerative potential remains an unmet goal. Thus, the aim of this study was the generation and in vitro biomechanical evaluation of a novel cardiovascular patch composed of pressure-compacted fibrin with embedded spider silk cocoons. Methods: Fibrin-based patches were cast in a customized circular mold. One cocoon of Nephila odulis spider silk was embedded per patch during the casting process. After polymerization, the fibrin clot was compacted by 2 kg weight for 30 min resulting in thickness reduction from up to 2 cm to <1 mm. Tensile strength and burst pressure was determined after 0 weeks and 14 weeks of storage. A sewing strength test and a long-term load test were performed using a customized device to exert physiological pulsatile stretching of a silicon surface on which the patch had been sutured. Results: Fibrin patches resisted supraphysiological pressures of well over 2000 mmHg. Embedding of spider silk increased tensile force 1.8-fold and tensile strength 1.45-fold (p <.001), resulting in a final strength of 1.07 MPa and increased sewing strength. Storage for 14 weeks decreased tensile strength, but not significantly and suturing properties of the spider silk patches were satisfactory. The long-term load test indicated that the patches were stable for 4 weeks although slight reduction in patch material was observed. Conclusion: The combination of compacted fibrin matrices and spider silk cocoons may represent a feasible concept to generate stable and biocompatible cardiovascular patches with regenerative potential.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Ingenieurwesen (insg.)
- Biomedizintechnik
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in: Journal of Biomaterials Applications, Jahrgang 36, Nr. 6, 01.2022, S. 1126-1136.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Pressure-compacted and spider silk–reinforced fibrin demonstrates sufficient biomechanical stability as cardiac patch in vitro
AU - Bobylev, Dmitry
AU - Wilhelmi, Mathias
AU - Lau, Skadi
AU - Klingenberg, Melanie
AU - Mlinaric, Markus
AU - Petená, Elena
AU - Helms, Florian
AU - Hassel, Thomas
AU - Haverich, Axel
AU - Horke, Alexander
AU - Böer, Ulrike
N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded by Kinderherzen e.V. (Fördergemeinschaft Deutsche Kinderherzzentren, Bonn, Germany) and the CORTISS Foundation (CORTISS Herz-und Gewebeforschungs GmbH, Hannover, Germany).
PY - 2022/1
Y1 - 2022/1
N2 - Objective: The generation of bio-/hemocompatible cardiovascular patches with sufficient stability and regenerative potential remains an unmet goal. Thus, the aim of this study was the generation and in vitro biomechanical evaluation of a novel cardiovascular patch composed of pressure-compacted fibrin with embedded spider silk cocoons. Methods: Fibrin-based patches were cast in a customized circular mold. One cocoon of Nephila odulis spider silk was embedded per patch during the casting process. After polymerization, the fibrin clot was compacted by 2 kg weight for 30 min resulting in thickness reduction from up to 2 cm to <1 mm. Tensile strength and burst pressure was determined after 0 weeks and 14 weeks of storage. A sewing strength test and a long-term load test were performed using a customized device to exert physiological pulsatile stretching of a silicon surface on which the patch had been sutured. Results: Fibrin patches resisted supraphysiological pressures of well over 2000 mmHg. Embedding of spider silk increased tensile force 1.8-fold and tensile strength 1.45-fold (p <.001), resulting in a final strength of 1.07 MPa and increased sewing strength. Storage for 14 weeks decreased tensile strength, but not significantly and suturing properties of the spider silk patches were satisfactory. The long-term load test indicated that the patches were stable for 4 weeks although slight reduction in patch material was observed. Conclusion: The combination of compacted fibrin matrices and spider silk cocoons may represent a feasible concept to generate stable and biocompatible cardiovascular patches with regenerative potential.
AB - Objective: The generation of bio-/hemocompatible cardiovascular patches with sufficient stability and regenerative potential remains an unmet goal. Thus, the aim of this study was the generation and in vitro biomechanical evaluation of a novel cardiovascular patch composed of pressure-compacted fibrin with embedded spider silk cocoons. Methods: Fibrin-based patches were cast in a customized circular mold. One cocoon of Nephila odulis spider silk was embedded per patch during the casting process. After polymerization, the fibrin clot was compacted by 2 kg weight for 30 min resulting in thickness reduction from up to 2 cm to <1 mm. Tensile strength and burst pressure was determined after 0 weeks and 14 weeks of storage. A sewing strength test and a long-term load test were performed using a customized device to exert physiological pulsatile stretching of a silicon surface on which the patch had been sutured. Results: Fibrin patches resisted supraphysiological pressures of well over 2000 mmHg. Embedding of spider silk increased tensile force 1.8-fold and tensile strength 1.45-fold (p <.001), resulting in a final strength of 1.07 MPa and increased sewing strength. Storage for 14 weeks decreased tensile strength, but not significantly and suturing properties of the spider silk patches were satisfactory. The long-term load test indicated that the patches were stable for 4 weeks although slight reduction in patch material was observed. Conclusion: The combination of compacted fibrin matrices and spider silk cocoons may represent a feasible concept to generate stable and biocompatible cardiovascular patches with regenerative potential.
KW - cardiovascular patch
KW - Congenital heart disease
KW - fibrin scaffold
KW - load test
KW - sewing test
KW - spider silk cocoons
KW - tensile strength
UR - http://www.scopus.com/inward/record.url?scp=85116520216&partnerID=8YFLogxK
U2 - 10.1177/08853282211046800
DO - 10.1177/08853282211046800
M3 - Article
C2 - 34617818
AN - SCOPUS:85116520216
VL - 36
SP - 1126
EP - 1136
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
SN - 0885-3282
IS - 6
ER -