Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 27046 |
| Seitenumfang | 11 |
| Fachzeitschrift | Scientific reports |
| Jahrgang | 14 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 7 Nov. 2024 |
Abstract
In this work, we report on the application of the polarization modulated spectroscopic ellipsometry-based surface plasmon resonance method for sensitive detection of microorganisms in Kretschmann configuration. So far, rotating analyzer and single wavelength polarization modulation methods have widely been investigated for phase sensitive surface plasmon resonance measurement. In this study, a much simpler optical setup relying on fast electro-optic phase modulator crystals is introduced for bacteria detection. A beta barium borate crystal connected to a function generator is adapted for generating phase shifts in the millisecond regime to extract the ellipsometric angles (Ψ and Δ) under the surface plasmon resonance condition. For detection, the gold surface was functionalized with anti-Escherichia coli antibodies, and E. coli K12 was attached to them. We show that polarization modulated spectroscopic ellipsometry achieves a refractive index resolution in the order of 10-5 RIU, and a limit of detection of 102 CFU/mL for E. coli K12 which is compatible with other surface plasmon resonance based phase sensitive methods with more complex detection concepts. As a follow-up step, an optical model can be developed to enhance this biosensor’s performance, and applications for sorting and detecting other biological targets will be investigated.
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in: Scientific reports, Jahrgang 14, Nr. 1, 27046, 07.11.2024.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Polarization modulated spectroscopic ellipsometry-based surface plasmon resonance biosensor for E. coli K12 detection
AU - Zangenehzadeh, Soraya
AU - Agocs, Emil
AU - Schröder, Fenja
AU - Amroun, Nassima
AU - Biedendieck, Rebekka
AU - Jahn, Dieter
AU - Günther, Axel
AU - Zheng, Lei
AU - Roth, Bernhard
AU - Johannes, Hans Hermann
AU - Kowalsky, Wolfgang
N1 - Publisher Copyright: © The Author(s) 2024.
PY - 2024/11/7
Y1 - 2024/11/7
N2 - In this work, we report on the application of the polarization modulated spectroscopic ellipsometry-based surface plasmon resonance method for sensitive detection of microorganisms in Kretschmann configuration. So far, rotating analyzer and single wavelength polarization modulation methods have widely been investigated for phase sensitive surface plasmon resonance measurement. In this study, a much simpler optical setup relying on fast electro-optic phase modulator crystals is introduced for bacteria detection. A beta barium borate crystal connected to a function generator is adapted for generating phase shifts in the millisecond regime to extract the ellipsometric angles (Ψ and Δ) under the surface plasmon resonance condition. For detection, the gold surface was functionalized with anti-Escherichia coli antibodies, and E. coli K12 was attached to them. We show that polarization modulated spectroscopic ellipsometry achieves a refractive index resolution in the order of 10-5 RIU, and a limit of detection of 102 CFU/mL for E. coli K12 which is compatible with other surface plasmon resonance based phase sensitive methods with more complex detection concepts. As a follow-up step, an optical model can be developed to enhance this biosensor’s performance, and applications for sorting and detecting other biological targets will be investigated.
AB - In this work, we report on the application of the polarization modulated spectroscopic ellipsometry-based surface plasmon resonance method for sensitive detection of microorganisms in Kretschmann configuration. So far, rotating analyzer and single wavelength polarization modulation methods have widely been investigated for phase sensitive surface plasmon resonance measurement. In this study, a much simpler optical setup relying on fast electro-optic phase modulator crystals is introduced for bacteria detection. A beta barium borate crystal connected to a function generator is adapted for generating phase shifts in the millisecond regime to extract the ellipsometric angles (Ψ and Δ) under the surface plasmon resonance condition. For detection, the gold surface was functionalized with anti-Escherichia coli antibodies, and E. coli K12 was attached to them. We show that polarization modulated spectroscopic ellipsometry achieves a refractive index resolution in the order of 10-5 RIU, and a limit of detection of 102 CFU/mL for E. coli K12 which is compatible with other surface plasmon resonance based phase sensitive methods with more complex detection concepts. As a follow-up step, an optical model can be developed to enhance this biosensor’s performance, and applications for sorting and detecting other biological targets will be investigated.
KW - E. coli bacteria
KW - Electro-optic polarization modulation
KW - Spectroscopic ellipsometry
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=85209131110&partnerID=8YFLogxK
U2 - 10.1038/s41598-024-78535-8
DO - 10.1038/s41598-024-78535-8
M3 - Article
C2 - 39511384
AN - SCOPUS:85209131110
VL - 14
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
IS - 1
M1 - 27046
ER -