Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Plasmonics in Biology and Medicine VI |
| Publikationsstatus | Veröffentlicht - 18 Feb. 2009 |
| Veranstaltung | Plasmonics in Biology and Medicine VI - San Jose, CA, USA / Vereinigte Staaten Dauer: 26 Jan. 2009 → 27 Jan. 2009 |
Publikationsreihe
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Band | 7192 |
| ISSN (Print) | 1605-7422 |
Abstract
Investigation on the interaction of small particles, e.g. gold nanoparticles with light is a current field of high interest. As light can be absorbed, enhanced or scattered by the nanoparticles a wide variety of possible applications become possible. If the electrons of such a nanoparticles oscillate with the incident light, plasmon resonances occur. Provided that these particles are brought very close to a cell, the cell membrane gets perforated due to the laser induced effect. We investigate nanoparticle mediated laser perforation as an alternative technique for cell transfection. By using weakly focussed femtosecond laser pulses, 150 nm gold particles were stimulated to perforate the cell membrane. Through the perforated area of the membrane macromolecules e.g. DNA are able to enter the cell. By this technique GFSHR-17 rat cells were successfully transfected with GFP vector and the dependence on laser parameters and concentration were studied. Even after 48 hours after manipulation the transfected cells show no indications of apoptosis or necrosis. This technique allows the transfection of cells by opto-perforation without the need of tight focusing conditions and single cell targeting- opening the way for a wide field of applications.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Medizin (insg.)
- Radiologie, Nuklearmedizin und Bildgebung
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Plasmonics in Biology and Medicine VI. 2009. 71920U (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 7192).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Plasmonic perforation of living cells using ultrashort laser pulses and gold nanoparticles
AU - Schomaker, Markus
AU - Baumgart, Judith
AU - Ngezahayo, Anaclet
AU - Bullerdiek, Jörn
AU - Nolte, Ingo
AU - Murua Escobar, Hugo
AU - Lubatschowski, Holger
AU - Heisterkamp, Alexander
PY - 2009/2/18
Y1 - 2009/2/18
N2 - Investigation on the interaction of small particles, e.g. gold nanoparticles with light is a current field of high interest. As light can be absorbed, enhanced or scattered by the nanoparticles a wide variety of possible applications become possible. If the electrons of such a nanoparticles oscillate with the incident light, plasmon resonances occur. Provided that these particles are brought very close to a cell, the cell membrane gets perforated due to the laser induced effect. We investigate nanoparticle mediated laser perforation as an alternative technique for cell transfection. By using weakly focussed femtosecond laser pulses, 150 nm gold particles were stimulated to perforate the cell membrane. Through the perforated area of the membrane macromolecules e.g. DNA are able to enter the cell. By this technique GFSHR-17 rat cells were successfully transfected with GFP vector and the dependence on laser parameters and concentration were studied. Even after 48 hours after manipulation the transfected cells show no indications of apoptosis or necrosis. This technique allows the transfection of cells by opto-perforation without the need of tight focusing conditions and single cell targeting- opening the way for a wide field of applications.
AB - Investigation on the interaction of small particles, e.g. gold nanoparticles with light is a current field of high interest. As light can be absorbed, enhanced or scattered by the nanoparticles a wide variety of possible applications become possible. If the electrons of such a nanoparticles oscillate with the incident light, plasmon resonances occur. Provided that these particles are brought very close to a cell, the cell membrane gets perforated due to the laser induced effect. We investigate nanoparticle mediated laser perforation as an alternative technique for cell transfection. By using weakly focussed femtosecond laser pulses, 150 nm gold particles were stimulated to perforate the cell membrane. Through the perforated area of the membrane macromolecules e.g. DNA are able to enter the cell. By this technique GFSHR-17 rat cells were successfully transfected with GFP vector and the dependence on laser parameters and concentration were studied. Even after 48 hours after manipulation the transfected cells show no indications of apoptosis or necrosis. This technique allows the transfection of cells by opto-perforation without the need of tight focusing conditions and single cell targeting- opening the way for a wide field of applications.
KW - Cell manipulation
KW - Nanoparticles
KW - Plasmon resonance
KW - Ultrashort laser pulses
UR - http://www.scopus.com/inward/record.url?scp=66649103000&partnerID=8YFLogxK
U2 - 10.1117/12.809316
DO - 10.1117/12.809316
M3 - Conference contribution
AN - SCOPUS:66649103000
SN - 9780819474384
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Plasmonics in Biology and Medicine VI
T2 - Plasmonics in Biology and Medicine VI
Y2 - 26 January 2009 through 27 January 2009
ER -