Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1819-1825 |
| Seitenumfang | 7 |
| Fachzeitschrift | Biotechnology letters |
| Jahrgang | 23 |
| Ausgabenummer | 22 |
| Publikationsstatus | Veröffentlicht - Nov. 2001 |
| Extern publiziert | Ja |
Abstract
Production of recombinant proteins often interferes with the physiology of the host organism by causing stress responses. In recombinant Escherichia coli, the cellular content of ColEl-derived plasmids and, consequently, the synthesis of the constitutively synthesized plasmid-encoded proteins generally increases after a temperature upshift. Simultaneous induction of inducible recombinant proteins that are synthesized at high levels and tend to form inclusion bodies, however, attenuates the plasmid amplification. This phenomenon was observed using temperature- as well as IPTG-inducible expression systems. Thus, high-level recombinant gene expression in connection with inclusion body formation does not only interfere with host cell but also with plasmid-related functions.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Biotechnology letters, Jahrgang 23, Nr. 22, 11.2001, S. 1819-1825.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Plasmid amplification in Escherichia coli after temperature upshift is impaired by induction of recombinant protein synthesis
AU - Hoffmann, Frank
AU - Rinas, Ursula
PY - 2001/11
Y1 - 2001/11
N2 - Production of recombinant proteins often interferes with the physiology of the host organism by causing stress responses. In recombinant Escherichia coli, the cellular content of ColEl-derived plasmids and, consequently, the synthesis of the constitutively synthesized plasmid-encoded proteins generally increases after a temperature upshift. Simultaneous induction of inducible recombinant proteins that are synthesized at high levels and tend to form inclusion bodies, however, attenuates the plasmid amplification. This phenomenon was observed using temperature- as well as IPTG-inducible expression systems. Thus, high-level recombinant gene expression in connection with inclusion body formation does not only interfere with host cell but also with plasmid-related functions.
AB - Production of recombinant proteins often interferes with the physiology of the host organism by causing stress responses. In recombinant Escherichia coli, the cellular content of ColEl-derived plasmids and, consequently, the synthesis of the constitutively synthesized plasmid-encoded proteins generally increases after a temperature upshift. Simultaneous induction of inducible recombinant proteins that are synthesized at high levels and tend to form inclusion bodies, however, attenuates the plasmid amplification. This phenomenon was observed using temperature- as well as IPTG-inducible expression systems. Thus, high-level recombinant gene expression in connection with inclusion body formation does not only interfere with host cell but also with plasmid-related functions.
KW - Escherichia coli
KW - Inclusion bodies
KW - Plasmid amplification
KW - Recombinant protein
UR - http://www.scopus.com/inward/record.url?scp=0035180274&partnerID=8YFLogxK
U2 - 10.1023/A:1012718200638
DO - 10.1023/A:1012718200638
M3 - Article
AN - SCOPUS:0035180274
VL - 23
SP - 1819
EP - 1825
JO - Biotechnology letters
JF - Biotechnology letters
SN - 0141-5492
IS - 22
ER -