Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 569-583 |
| Seitenumfang | 15 |
| Fachzeitschrift | Plant molecular biology reporter |
| Jahrgang | 33 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 16 Juni 2015 |
Abstract
Plant defense responses are made up of broad plant defense mechanisms that involve an integrated signaling pathway. Powdery mildew caused by Erysiphe pisi is one of the most important diseases of pea. However, the mechanisms and pathways involved in the resistance against E. pisi are yet undiscovered. We studied the transcriptome of two Medicago truncatula genotypes, the powdery mildew susceptible commercial variety Parabinga and the resistant accession SA1306, at 4 and 12 h after E. pisi infection, using Mt16kOLI1 microarrays. Four hundred and forty six probes were differentially expressed between the two M. truncatula genotypes along the time points studied. RNA accumulation patterns suggest that the most prominent responses to pathogen infection occur at early infection stages. Most of the regulated genes are related to cell wall reinforcement, flavonoid, and phenylpropanoid biosynthesis. In addition, pathogenesis-related proteins and signaling pathways controlled by jasmonic acid and salicylic acid were found to be regulated during pathogen infection. This study provides the first comprehensive view of the genes and pathways activated in the E. pisi/M. truncatula pathosystem, allowing the identification of targets against this important disease.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: Plant molecular biology reporter, Jahrgang 33, Nr. 3, 16.06.2015, S. 569-583.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Plant Defense Responses in Medicago truncatula Unveiled by Microarray Analysis
AU - Curto, Miguel
AU - Krajinski, Franziska
AU - Küster, Helge
AU - Rubiales, Diego
N1 - Funding information: This work was supported by the Grain Legumes Integrated Project (FP6-2002-FOOD-1-506223) and Spanish project AGL2011-22524. M. Curto was funded by Spanish Research Training programme (FPI). Drs. E. Prats and S. Fondevilla are thanked for providing help with microscopy assessments. We are grateful to Carolina Johnstone for grammatical review. Special thanks to Bielefeld University Center for Biotechnology for providing microarray platform, hybridization facilities, and bioinformatics support.
PY - 2015/6/16
Y1 - 2015/6/16
N2 - Plant defense responses are made up of broad plant defense mechanisms that involve an integrated signaling pathway. Powdery mildew caused by Erysiphe pisi is one of the most important diseases of pea. However, the mechanisms and pathways involved in the resistance against E. pisi are yet undiscovered. We studied the transcriptome of two Medicago truncatula genotypes, the powdery mildew susceptible commercial variety Parabinga and the resistant accession SA1306, at 4 and 12 h after E. pisi infection, using Mt16kOLI1 microarrays. Four hundred and forty six probes were differentially expressed between the two M. truncatula genotypes along the time points studied. RNA accumulation patterns suggest that the most prominent responses to pathogen infection occur at early infection stages. Most of the regulated genes are related to cell wall reinforcement, flavonoid, and phenylpropanoid biosynthesis. In addition, pathogenesis-related proteins and signaling pathways controlled by jasmonic acid and salicylic acid were found to be regulated during pathogen infection. This study provides the first comprehensive view of the genes and pathways activated in the E. pisi/M. truncatula pathosystem, allowing the identification of targets against this important disease.
AB - Plant defense responses are made up of broad plant defense mechanisms that involve an integrated signaling pathway. Powdery mildew caused by Erysiphe pisi is one of the most important diseases of pea. However, the mechanisms and pathways involved in the resistance against E. pisi are yet undiscovered. We studied the transcriptome of two Medicago truncatula genotypes, the powdery mildew susceptible commercial variety Parabinga and the resistant accession SA1306, at 4 and 12 h after E. pisi infection, using Mt16kOLI1 microarrays. Four hundred and forty six probes were differentially expressed between the two M. truncatula genotypes along the time points studied. RNA accumulation patterns suggest that the most prominent responses to pathogen infection occur at early infection stages. Most of the regulated genes are related to cell wall reinforcement, flavonoid, and phenylpropanoid biosynthesis. In addition, pathogenesis-related proteins and signaling pathways controlled by jasmonic acid and salicylic acid were found to be regulated during pathogen infection. This study provides the first comprehensive view of the genes and pathways activated in the E. pisi/M. truncatula pathosystem, allowing the identification of targets against this important disease.
KW - Erysiphe pisi
KW - Legume
KW - Medicago truncatula
KW - Microarray
KW - Pea
UR - http://www.scopus.com/inward/record.url?scp=84936944279&partnerID=8YFLogxK
U2 - 10.1007/s11105-014-0770-9
DO - 10.1007/s11105-014-0770-9
M3 - Article
AN - SCOPUS:84936944279
VL - 33
SP - 569
EP - 583
JO - Plant molecular biology reporter
JF - Plant molecular biology reporter
SN - 0735-9640
IS - 3
ER -