Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Pei Pei Chong
  • Sylvia M. Podmore
  • Helen M. Kieser
  • Matthias Redenbach
  • Kürsad Turgay
  • Mohamed Marahiel
  • David A. Hopwood
  • Colin P. Smith

Externe Organisationen

  • University of Manchester
  • John Innes Centre
  • Technische Universität Kaiserslautern
  • Philipps-Universität Marburg
  • Public Health Research Institute - New York
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)193-199
Seitenumfang7
FachzeitschriftMicrobiology
Jahrgang144
Ausgabenummer1
PublikationsstatusVeröffentlicht - Jan. 1998
Extern publiziertJa

Abstract

Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single ~ 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.

ASJC Scopus Sachgebiete

Zitieren

Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2). / Chong, Pei Pei; Podmore, Sylvia M.; Kieser, Helen M. et al.
in: Microbiology, Jahrgang 144, Nr. 1, 01.1998, S. 193-199.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Chong, P. P., Podmore, S. M., Kieser, H. M., Redenbach, M., Turgay, K., Marahiel, M., Hopwood, D. A., & Smith, C. P. (1998). Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2). Microbiology, 144(1), 193-199. https://doi.org/10.1099/00221287-144-1-193
Chong PP, Podmore SM, Kieser HM, Redenbach M, Turgay K, Marahiel M et al. Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2). Microbiology. 1998 Jan;144(1):193-199. doi: 10.1099/00221287-144-1-193
Download
@article{9e371464c15e405fa72ed0cd34730b54,
title = "Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)",
abstract = "Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single ~ 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.",
keywords = "Antibiotic biosynthesis, Gene disruption, Lipopeptide, Peptide synthetase, Streptomyces coelicolor",
author = "Chong, {Pei Pei} and Podmore, {Sylvia M.} and Kieser, {Helen M.} and Matthias Redenbach and K{\"u}rsad Turgay and Mohamed Marahiel and Hopwood, {David A.} and Smith, {Colin P.}",
note = "Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "1998",
month = jan,
doi = "10.1099/00221287-144-1-193",
language = "English",
volume = "144",
pages = "193--199",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Microbiology Society",
number = "1",

}

Download

TY - JOUR

T1 - Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

AU - Chong, Pei Pei

AU - Podmore, Sylvia M.

AU - Kieser, Helen M.

AU - Redenbach, Matthias

AU - Turgay, Kürsad

AU - Marahiel, Mohamed

AU - Hopwood, David A.

AU - Smith, Colin P.

N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 1998/1

Y1 - 1998/1

N2 - Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single ~ 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.

AB - Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single ~ 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.

KW - Antibiotic biosynthesis

KW - Gene disruption

KW - Lipopeptide

KW - Peptide synthetase

KW - Streptomyces coelicolor

UR - http://www.scopus.com/inward/record.url?scp=0031963288&partnerID=8YFLogxK

U2 - 10.1099/00221287-144-1-193

DO - 10.1099/00221287-144-1-193

M3 - Article

C2 - 9537762

AN - SCOPUS:0031963288

VL - 144

SP - 193

EP - 199

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 1

ER -