TY - JOUR

T1 - Photosensitive poly-l-lysine/heparin interpolyelectrolyte complexes for delivery of genetic drugs

AU - Korzhikov-Vlakh, Viktor

AU - Katernuk, Iuliia

AU - Pilipenko, Iuliia

AU - Lavrentieva, Antonina

AU - Guryanov, Ivan

AU - Sharoyko, Vladimir

AU - Manshina, Alina A.

AU - Tennikova, Tatiana B.

N1 - Funding information: The financial support was provided by Russian Science Foundation (project no. 19-73-10045). Ms. I. Katernuk is grateful to the German-Russian Interdisciplinary Science Center (G-RISC) for the supporting one-month internship (project C-2019a-2) at the Institute of Technical Chemistry, Leibniz University Hannover (Hannover, Germany). The authors are grateful to: Arto Urtti and Marika Rupponen, University of Eastern Finland, as well as Thomas Scheper, Institute of Technical Chemistry, Leibniz University for valuable discussions and help. The authors also would like to thank the Magnetic Resonance Research Centre and Centre for Molecular and Cell Technologies of St. Petersburg State University for NMR and TEM investigations, respectively Funding: The financial support was provided by Russian Science Foundation (project no. 19-73-10045). Ms. I. Katernuk is grateful to the German–Russian Interdisciplinary Science Center (G-RISC) for the supporting one-month internship (project C-2019a-2) at the Institute of Technical Chemistry, Leibniz University Hannover (Hannover, Germany).

PY - 2020/5/8

Y1 - 2020/5/8

N2 - Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions-such as model oligonucleotide, siRNA, and pDNA-were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.

AB - Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions-such as model oligonucleotide, siRNA, and pDNA-were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.

KW - Heparin

KW - Interpolyelectrolyte complex

KW - PDNA

KW - Photosensitive

KW - Poly-l-lysine

KW - SiRNA

UR - http://www.scopus.com/inward/record.url?scp=85085328279&partnerID=8YFLogxK

U2 - 10.3390/POLYM12051077

DO - 10.3390/POLYM12051077

M3 - Article

C2 - 32397208

AN - SCOPUS:85085328279

VL - 12

JO - Polymers

JF - Polymers

SN - 2073-4360

IS - 5

M1 - 1077

ER -