Details
Originalsprache | Englisch |
---|---|
Seiten (von - bis) | 97-107 |
Seitenumfang | 11 |
Fachzeitschrift | Journal of Plastic, Reconstructive and Aesthetic Surgery |
Jahrgang | 64 |
Ausgabenummer | 1 |
Publikationsstatus | Veröffentlicht - 21 Apr. 2010 |
Abstract
Background: Ablative procedures that are used for the improvement of a degenerative process that leads to a loss of skin elasticity and integrity, injure or destroy the epidermis and its basement membrane and lead to fibrosis of the papillary dermis. It was recently shown in clinical and laboratory trials that percutaneous collagen induction (PCI) by multiple needle application is a method for safely treating wrinkles and scars and smoothening the skin without the risk of dyspigmentation. In our study, we describe the effect of PCI on epidermal thickness and the induction of genes relevant for regenerative processes in the skin in a small animal model. Methods: The purpose of this study in a rat model was to determine the effects of PCI on the skin both qualitatively and quantitatively. The epidermal and dermal changes were observed by histology and immunofluorescence. The changes in gene expression were measured by array analysis for cytokines, such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF)-7, epidermal growth factor (EGF) and extracellular matrix molecules such as collagen type I and type III. Results: The present study showed that PCI with topical vitamins resulted in a 140% increase in epidermal thickness; an increase in gene and protein expression of collagen I, glycosaminoglycans (GAGs) and growth factors such as VEGF, EGF and FGF7. The collagen fibre bundles were increased, thickened, and more loosely woven in both the papillary and reticular dermis. Conclusion: We were able to show that PCI modulates gene expression in skin of those genes that are relevant for extracellular matrix remodelling.
ASJC Scopus Sachgebiete
- Medizin (insg.)
- Chirurgie
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in: Journal of Plastic, Reconstructive and Aesthetic Surgery, Jahrgang 64, Nr. 1, 21.04.2010, S. 97-107.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Percutaneous collagen induction - regeneration in place of cicatrisation?
AU - Aust, M. C.
AU - Reimers, K.
AU - Kaplan, H. M.
AU - Stahl, F.
AU - Repenning, C.
AU - Scheper, T.
AU - Jahn, S.
AU - Schwaiger, N.
AU - Ipaktchi, R.
AU - Redeker, J.
AU - Altintas, M. A.
AU - Vogt, P. M.
PY - 2010/4/21
Y1 - 2010/4/21
N2 - Background: Ablative procedures that are used for the improvement of a degenerative process that leads to a loss of skin elasticity and integrity, injure or destroy the epidermis and its basement membrane and lead to fibrosis of the papillary dermis. It was recently shown in clinical and laboratory trials that percutaneous collagen induction (PCI) by multiple needle application is a method for safely treating wrinkles and scars and smoothening the skin without the risk of dyspigmentation. In our study, we describe the effect of PCI on epidermal thickness and the induction of genes relevant for regenerative processes in the skin in a small animal model. Methods: The purpose of this study in a rat model was to determine the effects of PCI on the skin both qualitatively and quantitatively. The epidermal and dermal changes were observed by histology and immunofluorescence. The changes in gene expression were measured by array analysis for cytokines, such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF)-7, epidermal growth factor (EGF) and extracellular matrix molecules such as collagen type I and type III. Results: The present study showed that PCI with topical vitamins resulted in a 140% increase in epidermal thickness; an increase in gene and protein expression of collagen I, glycosaminoglycans (GAGs) and growth factors such as VEGF, EGF and FGF7. The collagen fibre bundles were increased, thickened, and more loosely woven in both the papillary and reticular dermis. Conclusion: We were able to show that PCI modulates gene expression in skin of those genes that are relevant for extracellular matrix remodelling.
AB - Background: Ablative procedures that are used for the improvement of a degenerative process that leads to a loss of skin elasticity and integrity, injure or destroy the epidermis and its basement membrane and lead to fibrosis of the papillary dermis. It was recently shown in clinical and laboratory trials that percutaneous collagen induction (PCI) by multiple needle application is a method for safely treating wrinkles and scars and smoothening the skin without the risk of dyspigmentation. In our study, we describe the effect of PCI on epidermal thickness and the induction of genes relevant for regenerative processes in the skin in a small animal model. Methods: The purpose of this study in a rat model was to determine the effects of PCI on the skin both qualitatively and quantitatively. The epidermal and dermal changes were observed by histology and immunofluorescence. The changes in gene expression were measured by array analysis for cytokines, such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF)-7, epidermal growth factor (EGF) and extracellular matrix molecules such as collagen type I and type III. Results: The present study showed that PCI with topical vitamins resulted in a 140% increase in epidermal thickness; an increase in gene and protein expression of collagen I, glycosaminoglycans (GAGs) and growth factors such as VEGF, EGF and FGF7. The collagen fibre bundles were increased, thickened, and more loosely woven in both the papillary and reticular dermis. Conclusion: We were able to show that PCI modulates gene expression in skin of those genes that are relevant for extracellular matrix remodelling.
KW - Collagen
KW - Percutaneous collagen induction
KW - Regeneration
KW - Skin-rejuvenation
UR - http://www.scopus.com/inward/record.url?scp=78650414136&partnerID=8YFLogxK
U2 - 10.1016/j.bjps.2010.03.038
DO - 10.1016/j.bjps.2010.03.038
M3 - Article
C2 - 20413357
AN - SCOPUS:78650414136
VL - 64
SP - 97
EP - 107
JO - Journal of Plastic, Reconstructive and Aesthetic Surgery
JF - Journal of Plastic, Reconstructive and Aesthetic Surgery
SN - 1748-6815
IS - 1
ER -