TY - JOUR

T1 - Penicillin G sensor based on penicillin amidase coupled to a field effect transistor

AU - Brand, U.

AU - Scheper, T.

AU - Schügerl, K.

N1 - Funding information: The authors thank M. Busch and 0. Thordsen for excellent technical assistance in some of the experiments, and R. Ferretti and D. Wilhelm, Institute for Semiconductor Technology, University of Hannover, for valuable discussions. The financial support of the Bundesministerium ftir Forschung und Technologie of the Federal Republic of Germany is gratefully acknowledged.

PY - 1989

Y1 - 1989

N2 - A penicillin sensor for the rapid determination of penicillin G was developed. The basic element of this biosensor is a hydrogen ion-sensitive field effect transistor (pH-FET). The pH-sensitive surface of this device is modified with an immobilized penicillin amidase membrane layer. The penicillin FETs have a response time below 90 s, a useful operating range of 0.3-30 mM penicillin G and a long-term stability at room temperature of up to 100 days. The effects of various parameters (pH, concentration of the buffer, temperature, stirring of the analyte) on the sensor signal are described. Additional studies on the dissolved enzyme and on the enzyme membrane layers immobilized on wafer samples proved the good long-term stability of the biochemical component used for sensor construction. The application of the penicillin G sensor to bioprocess monitoring is briefly discussed.

AB - A penicillin sensor for the rapid determination of penicillin G was developed. The basic element of this biosensor is a hydrogen ion-sensitive field effect transistor (pH-FET). The pH-sensitive surface of this device is modified with an immobilized penicillin amidase membrane layer. The penicillin FETs have a response time below 90 s, a useful operating range of 0.3-30 mM penicillin G and a long-term stability at room temperature of up to 100 days. The effects of various parameters (pH, concentration of the buffer, temperature, stirring of the analyte) on the sensor signal are described. Additional studies on the dissolved enzyme and on the enzyme membrane layers immobilized on wafer samples proved the good long-term stability of the biochemical component used for sensor construction. The application of the penicillin G sensor to bioprocess monitoring is briefly discussed.

UR - http://www.scopus.com/inward/record.url?scp=0024444260&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(00)80906-X

DO - 10.1016/S0003-2670(00)80906-X

M3 - Article

AN - SCOPUS:0024444260

VL - 226

SP - 87

EP - 97

JO - Analytica chimica acta

JF - Analytica chimica acta

SN - 0003-2670

IS - 1

ER -