TY - JOUR

T1 - Overlapping expression patterns and differential transcript levels of phosphate transporter genes in arbuscular mycorrhizal, Pi-fertilised and phytohormone-treated Medicago truncatula roots

AU - Grunwald, Ulf

AU - Guo, Wenbing

AU - Fischer, Kerstin

AU - Isayenkov, Stanislav

AU - Ludwig-Müller, Jutta

AU - Hause, Bettina

AU - Yan, Xiaolong

AU - Küster, Helge

AU - Franken, Philipp

N1 - Funding information: Acknowledgments The work was carried out in frame of the MolMyk programme funded by the German research foundation (DFG SPP 1084). The visit of Wenbing Guo at the IGZ was Wnanced by the German Federal Ministry of Food, Agriculture and Consumer Protection (BMVEL) and by the Chinese Ministry of Agriculture and Ministry of Science and Technology. Helge Küster acknowledges Wnancial support from the International Graduate School in Bioinfor-matics and Genome Research.

PY - 2009/4/1

Y1 - 2009/4/1

N2 - A microarray carrying 5,648 probes of Medicago truncatula root-expressed genes was screened in order to identify those that are specifically regulated by the arbuscular mycorrhizal (AM) fungus Gigaspora rosea, by Pi fertilisation or by the phytohormones abscisic acid and jasmonic acid. Amongst the identified genes, 21% showed a common induction and 31% a common repression between roots fertilised with Pi or inoculated with the AM fungus G. rosea, while there was no obvious overlap in the expression patterns between mycorrhizal and phytohormone-treated roots. Expression patterns were further studied by comparing the results with published data obtained from roots colonised by the AM fungi Glomus mosseae and Glomus intraradices, but only very few genes were identified as being commonly regulated by all three AM fungi. Analysis of Pi concentrations in plants colonised by either of the three AM fungi revealed that this could be due to the higher Pi levels in plants inoculated by G. rosea compared with the other two fungi, explaining that numerous genes are commonly regulated by the interaction with G. rosea and by phosphate. Differential gene expression in roots inoculated with the three AM fungi was further studied by expression analyses of six genes from the phosphate transporter gene family in M. truncatula. While MtPT4 was induced by all three fungi, the other five genes showed different degrees of repression mirroring the functional differences in phosphate nutrition by G. rosea, G. mosseae and G. intraradices.

AB - A microarray carrying 5,648 probes of Medicago truncatula root-expressed genes was screened in order to identify those that are specifically regulated by the arbuscular mycorrhizal (AM) fungus Gigaspora rosea, by Pi fertilisation or by the phytohormones abscisic acid and jasmonic acid. Amongst the identified genes, 21% showed a common induction and 31% a common repression between roots fertilised with Pi or inoculated with the AM fungus G. rosea, while there was no obvious overlap in the expression patterns between mycorrhizal and phytohormone-treated roots. Expression patterns were further studied by comparing the results with published data obtained from roots colonised by the AM fungi Glomus mosseae and Glomus intraradices, but only very few genes were identified as being commonly regulated by all three AM fungi. Analysis of Pi concentrations in plants colonised by either of the three AM fungi revealed that this could be due to the higher Pi levels in plants inoculated by G. rosea compared with the other two fungi, explaining that numerous genes are commonly regulated by the interaction with G. rosea and by phosphate. Differential gene expression in roots inoculated with the three AM fungi was further studied by expression analyses of six genes from the phosphate transporter gene family in M. truncatula. While MtPT4 was induced by all three fungi, the other five genes showed different degrees of repression mirroring the functional differences in phosphate nutrition by G. rosea, G. mosseae and G. intraradices.

KW - Abscisic acid

KW - Arbuscular mycorrhiza

KW - Gigaspora rosea

KW - Glomus intraradices

KW - Glomus mosseae

KW - Jasmonic acid

KW - Phosphate transporter genes

UR - http://www.scopus.com/inward/record.url?scp=63349098693&partnerID=8YFLogxK

U2 - 10.1007/s00425-008-0877-z

DO - 10.1007/s00425-008-0877-z

M3 - Article

C2 - 19169704

AN - SCOPUS:63349098693

VL - 229

SP - 1023

EP - 1034

JO - PLANTA

JF - PLANTA

SN - 0032-0935

IS - 5

ER -