TY - JOUR

T1 - Organic phosphorus availability shapes the diversity of phoD-harboring bacteria in agricultural soil

AU - Wei, Xiaomeng

AU - Hu, Yajun

AU - Cai, Guan

AU - Yao, Huaiying

AU - Ye, Jun

AU - Sun, Qi

AU - Veresoglou, Stavros D.

AU - Li, Yaying

AU - Zhu, Zhenke

AU - Guggenberger, Georg

AU - Chen, Xiangbi

AU - Su, Yirong

AU - Li, Yong

AU - Wu, Jinshui

AU - Ge, Tida

N1 - Funding Information: This study was supported by the National Key Research and Development program ( 2017YFD0800104 ), the National Natural Science Foundation of China ( 41601260, 41761134095 ); the  Natural Science Foundation of Hunan Province ( 2019JJ10003; 2019JJ30028 ); the Youth Innovation Team Project of the Institute of Subtropical Agriculture, Chinese Academy of Sciences ( 2017QNCXTD_GTD ) and the Hunan Province Base for Scientific and Technological Innovation Cooperation ( 2018WK4012 ). We thank anonymous reviewers for their careful and patient work that have helped to improve the quality of this manuscript.

PY - 2021/10

Y1 - 2021/10

N2 - In light of the limited resources of phosphorus (P) fertilizer, investigating the response of organic P (Po)-mineralizing microbial communities on the resource supply can be an avenue to optimize P recycling in agricultural systems. The alkaline phosphomonoesterase (alkaline PAse)-encoding gene PhoD is universally occurring in soil microorganisms. Here we collected 102 soil samples from Chinese agricultural fields to explore the effect of resource supply on the community of phoD-harboring bacteria. The relationships between the community diversity and soil organic carbon (SOC), total nitrogen (TN) and available Po concentration were fitted to the linear and quadric models suggested by the resource competition theory as well as the Michaelis-Menten model suggested by the metabolic theory of ecology. The results revealed that the response of phoD-harboring bacterial diversity to SOC and TN was likely related to the resource competition theory, with highest diversity at moderate SOC and TN concentration. In contrast, the phoD diversity increased with increasing available Po until the stationary value, which was consistent with the metabolic theory of ecology. Random forest models and multiple regression tree analyses identified the Po availability as the most important predictor on the variation of the phoD-harboring bacterial diversity and network topological features prior to the climate, soil texture, pH and all tested soil nutrient variables. This study highlights the critical role that Po plays in structuring phoD-harboring bacterial communities. Furthermore, for the first time, we correlated functional gene diversity to the corresponding enzymatic substrate availability from a metabolic theory perspective, confirming that the relationship follows the Michaelis-Menten model which was well known to predict the substrate regulation on the rate of enzymatic reactions.

AB - In light of the limited resources of phosphorus (P) fertilizer, investigating the response of organic P (Po)-mineralizing microbial communities on the resource supply can be an avenue to optimize P recycling in agricultural systems. The alkaline phosphomonoesterase (alkaline PAse)-encoding gene PhoD is universally occurring in soil microorganisms. Here we collected 102 soil samples from Chinese agricultural fields to explore the effect of resource supply on the community of phoD-harboring bacteria. The relationships between the community diversity and soil organic carbon (SOC), total nitrogen (TN) and available Po concentration were fitted to the linear and quadric models suggested by the resource competition theory as well as the Michaelis-Menten model suggested by the metabolic theory of ecology. The results revealed that the response of phoD-harboring bacterial diversity to SOC and TN was likely related to the resource competition theory, with highest diversity at moderate SOC and TN concentration. In contrast, the phoD diversity increased with increasing available Po until the stationary value, which was consistent with the metabolic theory of ecology. Random forest models and multiple regression tree analyses identified the Po availability as the most important predictor on the variation of the phoD-harboring bacterial diversity and network topological features prior to the climate, soil texture, pH and all tested soil nutrient variables. This study highlights the critical role that Po plays in structuring phoD-harboring bacterial communities. Furthermore, for the first time, we correlated functional gene diversity to the corresponding enzymatic substrate availability from a metabolic theory perspective, confirming that the relationship follows the Michaelis-Menten model which was well known to predict the substrate regulation on the rate of enzymatic reactions.

KW - Co-occurrence network

KW - Community diversity

KW - Organic phosphorus availability

KW - phoD-harboring bacteria

UR - http://www.scopus.com/inward/record.url?scp=85111801297&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2021.108364

DO - 10.1016/j.soilbio.2021.108364

M3 - Article

AN - SCOPUS:85111801297

VL - 161

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

M1 - 108364

ER -